Mouse Anti-COPE Recombinant Antibody (BR139) (V2LY-1206-LY760)

Name: Mouse Anti-COPE Recombinant Antibody (BR139)
SKU: V2LY-1206-LY760
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-COPE Recombinant Antibody (BR139) (V2LY-1206-LY760) in WB

Immunoblot analysis of THP-1 cells after CRISPR/Cas9-mediated genetic deletion of COPI-subunit proteins COPA, COPG1, COPD and COPE following treatment with STING inhibitor H-151 (2.5 µM). Results are shown as a representative of n = 3 independent repeats. ...View More

Steiner, A., Hrovat-Schaale, K., Prigione, I., Yu, C. H., Laohamonthonkul, P., Harapas, C. R., ... & Masters, S. L. (2022). Deficiency in coatomer complex I causes aberrant activation of STING signalling. Nature communications, 13(1), 2321.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

BR139

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

Amino acids 111-190 of human COPE.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

coatomer protein complex subunit epsilon

Entrez Gene ID

Human	11316
Mouse	59042
Rat	290659

UniProt ID

Human	O14579
Mouse	O89079
Rat	G3V8Q1

Function

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).

Biological Process

Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Intra-Golgi vesicle-mediated transport Source: UniProtKB
Protein transport Source: UniProtKB-KW
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome

Cellular Location

Cytoplasm; Golgi apparatus membrane; COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.

PTM

Phosphorylated by PKA.
Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.

Matsumura, T., Terada, J., Kinoshita, T., Sakurai, Y., Yahaba, M., Ema, R., ... & Hiwasa, T. (2017). Circulating anti-Coatomer protein complex subunit epsilon (COPE) autoantibodies as a potential biomarker for cardiovascular and cerebrovascular events in patients with obstructive sleep apnea. Journal of Clinical Sleep Medicine, 13(3), 393-400.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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