Mouse Anti-CREB3L2 Recombinant Antibody (CBFYC-2196) (V2LY-1206-LY1104)

Name: Mouse Anti-CREB3L2 Recombinant Antibody (CBFYC-2196)
SKU: V2LY-1206-LY1104
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2196

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 172-197 within an internal region of human CREB3L2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CAMP Responsive Element Binding Protein 3 Like 2

Entrez Gene ID

Human	64764
Mouse	208647
Rat	362339

UniProt ID

Human	Q70SY1
Mouse	Q8BH52
Rat	Q6QDP7

Function

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes (By similarity).

In a neuroblastoma cell line, protects cells from ER stress-induced death (PubMed:17178827).

In vitro activates transcription of target genes via direct binding to the CRE site (PubMed:17178827).

Biological Process

Cartilage development Source: UniProtKB
Chondrocyte differentiation Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Ensembl
Endoplasmic reticulum unfolded protein response Source: InterPro
Positive regulation of gene expression Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to endoplasmic reticulum stress Source: UniProtKB
Response to wounding Source: Ensembl

Cellular Location

Endoplasmic reticulum membrane. ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.
Processed cyclic AMP-responsive element-binding protein 3-like protein 2: Nucleus. Upon ER stress, translocated into the nucleus.

Involvement in disease

A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.

Topology

Cytoplasmic: 1-379
Helical: 380-400
Lumenal: 401-520

PTM

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).
N-glycosylated.
Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized.

Tomoishi, S., Fukushima, S., Shinohara, K., Katada, T., & Saito, K. (2017). CREB3L2-mediated expression of Sec23A/Sec24D is involved in hepatic stellate cell activation through ER-Golgi transport. Scientific reports, 7(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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