Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580)
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Published Data
Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580) in IHC
(A-E) Photomicrographs of immunostaining expression in rat spinal cord of CX3CR1 (streptavidin-biotin-peroxidase; Harris hematoxylin; 40×). (F-J) Photomicrographs of the immunohistochemical expression of CXCR4 (streptavidin-biotin-peroxidase; Harris hematoxylin; 40×).
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Drummond, I. S. A., de Oliveira, J. N. S., Niella, R. V., Silva, Á. J. C., de Oliveira, I. S., de Souza, S. S., ... & de Lavor, M. S. L. (2024). Evaluation of the Therapeutic Potential of Amantadine in a Vincristine-Induced Peripheral Neuropathy Model in Rats. Animals, 14(13), 1941. Distributed under Open Access license CC BY 4.0, without modification.
Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580) in IF
Prolonged anesthesia enhanced microglial chemotaxis. The iba1(green) positive microglia were colocalized with CX3CR1 (red) in the hippocampal CA1 region. Scale bar = 10 μm.
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Xu, F., Han, L., Wang, Y., Deng, D., Ding, Y., Zhao, S., ... & Chen, X. (2023). Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors. BMC medicine, 21(1), 7. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580) in IF
CX3CR1 signals and its related protein expressions. CX3CR1 fluorescence signals were semiquantified.
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Kang, J. Y., Baek, D. C., Son, C. G., & Lee, J. S. (2022). Succinum extracts inhibit microglial-derived neuroinflammation and depressive-like behaviors. Frontiers in Pharmacology, 13, 991243. Distributed under Open Access license CC BY 4.0, without modification.
Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580) in FC
Flow cytometry was used to assess total leukocytes (CD45+), macrophages (CD45+CD64+CD11b+CX3CR1+), DCs (CD45+CD64−CD11c+MHCII+), B cells (B220+), and CD4+ T cells in MLN and spleen from normal and BLM ± SB mice.
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Park, H. J., Jeong, O. Y., Chun, S. H., Cheon, Y. H., Kim, M., Kim, S., & Lee, S. I. (2021). Butyrate improves skin/lung fibrosis and intestinal dysbiosis in bleomycin-induced mouse models. International journal of molecular sciences, 22(5), 2765. Distributed under Open Access license CC BY 4.0, without modification.
Mouse Anti-CX3CR1 Recombinant Antibody (CBFYC-0517) (V2LY-0125-LY580) in IF
Expression of CD11b+CX3CR1+ macrophages in skin tissues from normal and bleomycin (BLM) ± sodium butyrate (SB) mice. BLM was injected subcutaneously for two weeks. SB was orally administered from two weeks before BLM injection and skin samples were then obtained. Representative immunofluorescent images of skin stained for DAPI (blue), CD11b (green), and CX3CR1 (red). Magnification, 400×. Scale bars = 100 µm. n = 6/group.
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Park, H. J., Jeong, O. Y., Chun, S. H., Cheon, Y. H., Kim, M., Kim, S., & Lee, S. I. (2021). Butyrate improves skin/lung fibrosis and intestinal dysbiosis in bleomycin-induced mouse models. International journal of molecular sciences, 22(5), 2765. Distributed under Open Access license CC BY 4.0, without modification.
Datasheet
Target
References
Q & As
Review & reward
Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
CBFYC-0517
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 131-200 mapping within an internal region of human CX3CR1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
| Application | Note |
| WB | 1:100-1:1,000 |
| IP | 1-2 μg per 100-500 μg of total protein (1 mL of cell lysate) |
| IF(ICC) | 1:50-1:500 |
| ELISA | 1:100-1:1,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation
Target
Full Name
C-X3-C Motif Chemokine Receptor 1
Entrez Gene ID
UniProt ID
Function
Receptor for the C-X3-C chemokine fractalkine (CX3CL1) present on many early leukocyte cells; CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:9390561, PubMed:9782118, PubMed:12055230, PubMed:23125415).
CX3CR1-CX3CL1 signaling mediates cell migratory functions (By similarity).
Responsible for the recruitment of natural killer (NK) cells to inflamed tissues (By similarity).
Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival (By similarity).
Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung (By similarity).
Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells (By similarity).
Acts as a negative regulator of angiogenesis, probably by promoting macrophage chemotaxis (PubMed:14581400, PubMed:18971423).
Plays a key role in brain microglia by regulating inflammatory response in the central nervous system (CNS) and regulating synapse maturation (By similarity).
Required to restrain the microglial inflammatory response in the CNS and the resulting parenchymal damage in response to pathological stimuli (By similarity).
Involved in brain development by participating to synaptic pruning, a natural process during which brain microglia eliminates extra synapses during postnatal development (By similarity).
Synaptic pruning by microglia is required to promote the maturation of circuit connectivity during brain development (By similarity).
Acts as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi (By similarity).
Expressed in lamina propria dendritic cells in the small intestine, which form transepithelial dendrites capable of taking up bacteria in order to provide defense against pathogenic bacteria (By similarity).
Required to initiate innate and adaptive immune responses against dissemination of commensal fungi (mycobiota) component of the gut: expressed in mononuclear phagocytes (MNPs) and acts by promoting induction of antifungal IgG antibodies response to confer protection against disseminated C.albicans or C.auris infection (PubMed:29326275).
Also acts as a receptor for C-C motif chemokine CCL26, inducing cell chemotaxis (PubMed:20974991).
Isoform 1:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein.
Isoform 2:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein (PubMed:14607932).
May have more potent HIV-1 coreceptothr activity than isoform 1 (PubMed:14607932).
Isoform 3:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein (PubMed:14607932).
May have more potent HIV-1 coreceptor activity than isoform 1 (PubMed:14607932).
CX3CR1-CX3CL1 signaling mediates cell migratory functions (By similarity).
Responsible for the recruitment of natural killer (NK) cells to inflamed tissues (By similarity).
Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival (By similarity).
Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung (By similarity).
Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells (By similarity).
Acts as a negative regulator of angiogenesis, probably by promoting macrophage chemotaxis (PubMed:14581400, PubMed:18971423).
Plays a key role in brain microglia by regulating inflammatory response in the central nervous system (CNS) and regulating synapse maturation (By similarity).
Required to restrain the microglial inflammatory response in the CNS and the resulting parenchymal damage in response to pathological stimuli (By similarity).
Involved in brain development by participating to synaptic pruning, a natural process during which brain microglia eliminates extra synapses during postnatal development (By similarity).
Synaptic pruning by microglia is required to promote the maturation of circuit connectivity during brain development (By similarity).
Acts as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi (By similarity).
Expressed in lamina propria dendritic cells in the small intestine, which form transepithelial dendrites capable of taking up bacteria in order to provide defense against pathogenic bacteria (By similarity).
Required to initiate innate and adaptive immune responses against dissemination of commensal fungi (mycobiota) component of the gut: expressed in mononuclear phagocytes (MNPs) and acts by promoting induction of antifungal IgG antibodies response to confer protection against disseminated C.albicans or C.auris infection (PubMed:29326275).
Also acts as a receptor for C-C motif chemokine CCL26, inducing cell chemotaxis (PubMed:20974991).
Isoform 1:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein.
Isoform 2:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein (PubMed:14607932).
May have more potent HIV-1 coreceptothr activity than isoform 1 (PubMed:14607932).
Isoform 3:
(Microbial infection) Acts as coreceptor with CD4 for HIV-1 virus envelope protein (PubMed:14607932).
May have more potent HIV-1 coreceptor activity than isoform 1 (PubMed:14607932).
Biological Process
Autocrine signaling Source: ARUK-UCL
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ARUK-UCL
Cell-cell signaling Source: ARUK-UCL
Cell chemotaxis Source: GO_Central
Cellular defense response Source: ProtInc
Cellular response to lipopolysaccharide Source: ARUK-UCL
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Central nervous system maturation Source: ARUK-UCL
Chemotaxis Source: ARUK-UCL
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: GO_Central
Innate immune response Source: ARUK-UCL
Leukocyte tethering or rolling Source: ARUK-UCL
Memory Source: Ensembl
Modulation of chemical synaptic transmission Source: ARUK-UCL
Multiple spine synapse organization, single dendrite Source: ARUK-UCL
Negative regulation of apoptotic signaling pathway Source: ARUK-UCL
Negative regulation of cell migration Source: Ensembl
Negative regulation of hippocampal neuron apoptotic process Source: ARUK-UCL
Negative regulation of interleukin-1 beta production Source: ARUK-UCL
Negative regulation of long-term synaptic potentiation Source: Ensembl
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: ARUK-UCL
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of I-kappaB phosphorylation Source: ARUK-UCL
Positive regulation of microglial cell migration Source: Ensembl
Positive regulation of neuroblast proliferation Source: ARUK-UCL
Positive regulation of neurogenesis Source: ARUK-UCL
Positive regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Positive regulation of protein kinase B signaling Source: ARUK-UCL
Regulation of microglial cell migration Source: ARUK-UCL
Regulation of neurogenesis Source: ARUK-UCL
Regulation of nitric oxide biosynthetic process Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Regulation of tumor necrosis factor production Source: ARUK-UCL
Response to ischemia Source: ARUK-UCL
Response to wounding Source: ProtInc
Social behavior Source: ARUK-UCL
Synapse maturation Source: ARUK-UCL
Synapse pruning Source: ARUK-UCL
Viral process Source: UniProtKB-KW
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ARUK-UCL
Cell-cell signaling Source: ARUK-UCL
Cell chemotaxis Source: GO_Central
Cellular defense response Source: ProtInc
Cellular response to lipopolysaccharide Source: ARUK-UCL
Cellular response to transforming growth factor beta stimulus Source: Ensembl
Central nervous system maturation Source: ARUK-UCL
Chemotaxis Source: ARUK-UCL
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: GO_Central
Innate immune response Source: ARUK-UCL
Leukocyte tethering or rolling Source: ARUK-UCL
Memory Source: Ensembl
Modulation of chemical synaptic transmission Source: ARUK-UCL
Multiple spine synapse organization, single dendrite Source: ARUK-UCL
Negative regulation of apoptotic signaling pathway Source: ARUK-UCL
Negative regulation of cell migration Source: Ensembl
Negative regulation of hippocampal neuron apoptotic process Source: ARUK-UCL
Negative regulation of interleukin-1 beta production Source: ARUK-UCL
Negative regulation of long-term synaptic potentiation Source: Ensembl
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: ARUK-UCL
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of I-kappaB phosphorylation Source: ARUK-UCL
Positive regulation of microglial cell migration Source: Ensembl
Positive regulation of neuroblast proliferation Source: ARUK-UCL
Positive regulation of neurogenesis Source: ARUK-UCL
Positive regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Positive regulation of protein kinase B signaling Source: ARUK-UCL
Regulation of microglial cell migration Source: ARUK-UCL
Regulation of neurogenesis Source: ARUK-UCL
Regulation of nitric oxide biosynthetic process Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Regulation of tumor necrosis factor production Source: ARUK-UCL
Response to ischemia Source: ARUK-UCL
Response to wounding Source: ProtInc
Social behavior Source: ARUK-UCL
Synapse maturation Source: ARUK-UCL
Synapse pruning Source: ARUK-UCL
Viral process Source: UniProtKB-KW
Cellular Location
Cell membrane
Involvement in disease
Macular degeneration, age-related, 12 (ARMD12):
A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Topology
Extracellular: 1-31
Helical: 32-59
Cytoplasmic: 60-69
Helical: 70-90
Extracellular: 91-103
Helical: 104-125
Cytoplasmic: 126-142
Helical: 143-167
Extracellular: 168-195
Helical: 196-215
Cytoplasmic: 216-231
Helical: 232-256
Extracellular: 257-273
Helical: 274-297
Cytoplasmic: 298-355
Helical: 32-59
Cytoplasmic: 60-69
Helical: 70-90
Extracellular: 91-103
Helical: 104-125
Cytoplasmic: 126-142
Helical: 143-167
Extracellular: 168-195
Helical: 196-215
Cytoplasmic: 216-231
Helical: 232-256
Extracellular: 257-273
Helical: 274-297
Cytoplasmic: 298-355
PTM
This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
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For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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