Mouse Anti-DBNL Recombinant Antibody (CBXS-5808) (V2LY-0125-LY1160)

Name: Mouse Anti-DBNL Recombinant Antibody (CBXS-5808)
SKU: V2LY-0125-LY1160
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXS-5808

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 100-186 mapping near the N-terminus of human HIP-55.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Drebrin Like

Entrez Gene ID

28988

UniProt ID

Q9UJU6

Function

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity).

May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.

Biological Process

Activation of JUN kinase activity Source: ProtInc
Adaptive immune response Source: UniProtKB-KW
Neuron projection morphogenesis Source: UniProtKB
Neutrophil degranulation Source: Reactome
Podosome assembly Source: UniProtKB
Positive regulation of axon extension Source: GO_Central
Positive regulation of dendritic spine morphogenesis Source: GO_Central
Postsynaptic actin cytoskeleton organization Source: GO_Central
Rac protein signal transduction Source: UniProtKB
Receptor-mediated endocytosis Source: InterPro
Regulation of actin filament polymerization Source: GO_Central
Ruffle assembly Source: InterPro
Synapse assembly Source: UniProtKB

Cellular Location

Cell membrane; Golgi apparatus membrane; Early endosome; Cytosol; Cytoskeleton; Lamellipodium; Ruffle; Cell cortex; Synapse; Perikaryon; Neuron projection; Clathrin-coated vesicle membrane; Podosome; Dendrite; Postsynaptic density. Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes.

PTM

Degraded by caspases during apoptosis.

Herrera, A., Menendez, A., Torroba, B., Ochoa, A., & Pons, S. (2021). Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity. Journal of Cell Biology, 220(6), e202007055.

Ghosh, A., Enderlein, J., & Butkevich, E. (2020). Dimerization of human drebrin-like protein governs its biological activity. Biochemistry, 59(16), 1553-1558.

Inoue, S., Hayashi, K., Fujita, K., Tagawa, K., Okazawa, H., Kubo, K. I., & Nakajima, K. (2019). Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex. Journal of Neuroscience, 39(4), 678-691.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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