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Mouse Anti-DCP1A Recombinant Antibody (2G10) (CBMAB-D0384-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Decapping MRNA 1A (DCP1A). The antibody can be used for immunoassay techniques, such as WB.
See all DCP1A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G10
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
DCP1A (NP_060873, 186 a.a. ~ 286 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Decapping MRNA 1A
Introduction
Decapping is a key step in general and regulated mRNA decay. DCP1A is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.
Entrez Gene ID
55802
UniProt ID
Q9NPI6
Alternative Names
Decapping MRNA 1A; Smad4-Interacting Transcriptional Co-Activator; Transcription Factor SMIF; SMIF; DCP1 Decapping Enzyme Homolog A (S. Cerevisiae); Putative Protein Product Of Nbla00360; DCP1 Decapping Enzyme-Like Protein A; DCP1 Decapping Enzyme Homolog A;
Function
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Biological Process
Deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
Deadenylation-independent decapping of nuclear-transcribed mRNA Source: GO_Central
Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
Positive regulation of catalytic activity Source: InterPro
Protein localization to cytoplasmic stress granule Source: AgBase
Regulation of mRNA stability Source: Reactome
Cellular Location
Nucleus; P-body. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524).

Ibayashi, M., Aizawa, R., & Tsukamoto, S. (2021). mRNA decapping factor Dcp1a is essential for embryonic growth in mice. Biochemical and Biophysical Research Communications, 555, 128-133.

Zhu, X., Chen, J., Tian, L., Zhou, Y., Xu, S., Long, S., ... & Xiao, S. (2020). Porcine deltacoronavirus nsp5 cleaves DCP1A To decrease Its antiviral activity. Journal of virology, 94(15), e02162-19.

Aeby, E., Lee, H. G., Lee, Y. W., Kriz, A., Del Rosario, B. C., Oh, H. J., ... & Lee, J. T. (2020). Decapping enzyme 1A breaks X-chromosome symmetry by controlling Tsix elongation and RNA turnover. Nature cell biology, 22(9), 1116-1129.

Wang, Y., Wang, H., Zhang, J., Chu, Z., Liu, P., Zhang, X., ... & Gu, X. (2020). Circ_0007031 serves as a sponge of miR-760 to regulate the growth and chemoradiotherapy resistance of colorectal cancer via regulating DCP1A. Cancer Management and Research, 12, 8465.

Wang, Y., Guo, W., Tang, K., Wang, Y., Zan, L., & Yang, W. (2019). Bta-miR-34b regulates milk fat biosynthesis by targeting mRNA decapping enzyme 1A (DCP1A) in cultured bovine mammary epithelial cells. Journal of animal science, 97(9), 3823-3831.

Wu, C., Liu, W., Ruan, T., Zhu, X., Tao, K., & Zhang, W. (2018). Overexpression of mRNA‑decapping enzyme 1a affects survival rate in colorectal carcinoma. Oncology Letters, 16(1), 1095-1100.

Tang, Y., Xie, C., Zhang, Y., Qin, Y., & Zhang, W. (2018). Overexpression of mRNA-decapping enzyme 1a predicts disease-specific survival in malignant melanoma. Melanoma Research, 28(1), 30-36.

Wu, C., Zhu, X., Tao, K., Liu, W., Ruan, T., Wan, W., ... & Zhang, W. (2018). MALAT1 promotes the colorectal cancer malignancy by increasing DCP1A expression and miR203 downregulation. Molecular Carcinogenesis, 57(10), 1421-1431.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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