Mouse Anti-DEK Recombinant Antibody (D0696) (V2LY-0425-LY168)

Name: Mouse Anti-DEK Recombinant Antibody (D0696)
SKU: V2LY-0425-LY168
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DEK Recombinant Antibody (D0696) (V2LY-0321-LY168-1) in IF

Confocal images of co-immunostained breast cancer model cell lines with DEK protein and H3K4me3, H3K9ac or H3K27ac histone modifications. Scale bars=5 μm. ...View More

Pierzynska-Mach, A., Cainero, I., Oneto, M., Ferrando-May, E., Lanzanò, L., & Diaspro, A. (2023). Imaging-based study demonstrates how the DEK nanoscale distribution differentially correlates with epigenetic marks in a breast cancer model. Scientific Reports, 13(1), 12749.

Mouse Anti-DEK Recombinant Antibody (D0696) (V2LY-0321-LY168-2) in WB

NuPAGE analysis showing polyphosphorylation induced shifts of DEK and MESD from cells sent for RNA-seq. β-Actin, a non-polyphosphorylated protein, was used as a loading control. Image is representative of n = 3. ...View More

Bondy-Chorney, E., Abramchuk, I., Nasser, R., Holinier, C., Denoncourt, A., Baijal, K., ... & Downey, M. (2020). A broad response to intracellular long-chain polyphosphate in human cells. Cell Reports, 33(4).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0696

Application

WB, IF, IP

Immunogen

Amino acids 19-169 of human DEK.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEK Proto-Oncogene

Entrez Gene ID

Human	7913
Mouse	110052
Rat	306817

UniProt ID

Human	P35659
Mouse	Q7TNV0
Rat	Q6AXS3

Function

Involved in chromatin organization.

Biological Process

Chromatin organization Source: UniProtKB-KW
Granulocyte differentiation Source: Reactome
Positive regulation of gene expression, epigenetic Source: Reactome
Regulation of double-strand break repair Source: MGI
Regulation of transcription by RNA polymerase II Source: Reactome
Signal transduction Source: ProtInc
Transcription by RNA polymerase II Source: ProtInc
Viral genome replication Source: ProtInc

Cellular Location

Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.

Involvement in disease

A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).

PTM

Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G1 phase, and weakens the binding of DEK to DNA.

Yang, M. Q., Bai, L. L., Wang, Z., Lei, L., Zheng, Y. W., Li, Z. H., ... & Xu, H. T. (2021). DEK is highly expressed in breast cancer and is associated with malignant phenotype and progression. Oncology Letters, 21(6), 1-9.

Cai, Y., Hao, Y., Xu, H., Chen, K., & Ren, B. (2021). Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non‑small cell lung cancer. Experimental and therapeutic medicine, 22(5), 1-9.

Yang, Y., Lin, H., Du, Z., Hu, R., Tang, Y., Liang, X., ... & Tan, Y. (2020). Imatinib therapy in acute myeloid leukemia with DEK‑NUP214 and FIP1L1‑PDGFRA rearrangement: A case report. Oncology Letters, 19(5), 3587-3592.

Liu, C. S., Zhou, Q., Zhang, Y. D., & Fu, Y. (2020). Long noncoding RNA SOX2OT maintains the stemness of pancreatic cancer cells by regulating DEK via interacting with miR-200a/141. European review for medical and pharmacological sciences, 24(23), 11991-11991.

Ishida, K., Nakashima, T., Shibata, T., Hara, A., & Tomita, H. (2020). Role of the DEK oncogene in the development of squamous cell carcinoma. International Journal of Clinical Oncology, 25(9), 1563-1569.

Matrka, M. C. (2017). Oncogenic Phenotypes Induced by Overexpression of the DEK Proto-oncogene (Doctoral dissertation, University of Cincinnati).

Xu, Y., Liang, Z., Li, C., Yang, Z., & Chen, L. (2017). LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells. Molecular Medicine Reports, 16(4), 4159-4164.

Nakashima, T., Tomita, H., Hirata, A., Ishida, K., Hisamatsu, K., Hatano, Y., ... & Hara, A. (2017). Promotion of cell proliferation by the proto‐oncogene DEK enhances oral squamous cell carcinogenesis through field cancerization. Cancer medicine, 6(10), 2424-2439.

Feng, T., Liu, Y., Li, C., Li, Z., & Cai, H. (2017). DEK proto-oncogene is highly expressed in astrocytic tumors and regulates glioblastoma cell proliferation and apoptosis. Tumor Biology, 39(7), 1010428317716248.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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