Mouse Anti-DHX16 Recombinant Antibody (D0847) (V2LY-0425-LY286)

Name: Mouse Anti-DHX16 Recombinant Antibody (D0847)
SKU: V2LY-0425-LY286
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DHX16 Recombinant Antibody (D0847) (V2LY-0425-LY286-1) in IP

DHX38 associates with multiple spliceosomal components.
DHX38WT-FLAG and DHX38G332D-FLAG were transiently expressed in HEK293 cells, immunoprecipitated and copurification of selected spliceosomal proteins monitored. Immunoprecipitation was performed in normal NET2 buffer containing 150 mM NaCl (A) or in stringent NET2 buffer containing 300 mM NaCl (B). ...View More

Obuća, M., Cvačková, Z., Kubovčiak, J., Kolář, M., & Staněk, D. (2022). Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity. Plos one, 17(4), e0265742.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0847

Application

WB, IP, IF

Immunogen

Amino acids 256-366 of human DDX16.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEAH (Asp-Glu-Ala-His) box polypeptide 16

Entrez Gene ID

8449

UniProt ID

O60231

Function

Required for pre-mRNA splicing as component of the spliceosome (PubMed:20423332, PubMed:20841358, PubMed:25296192, PubMed:29360106).

Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction.

Biological Process

mRNA splicing, via spliceosome Source: UniProtKB
RNA splicing Source: ProtInc

Cellular Location

Nucleus; Nucleoplasm

Involvement in disease

Neuromuscular oculoauditory syndrome (NMOAS):
An autosomal dominant neuromuscular disorder characterized by variable features including myopathy, neuropathy, hypotonia, joint contractures, growth delay, chorioretinal lacunae, sensorineuronal deafness, agenesis of the corpus callosum, and seizures.

Hage, A., Bharaj, P., van Tol, S., Giraldo, M. I., Gonzalez-Orozco, M., Valerdi, K. M., ... & Rajsbaum, R. (2022). The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity. Cell reports, 38(10), 110434.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-DHX16 Recombinant Antibody (2268C5_2a) (CAT#: CBMAB-D0847-YC)

Isotype control

For research use only. Not intended for any clinical use.

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