Mouse Anti-E2F2 Recombinant Antibody (CBFYE-0364) (V2LY-0425-LY1230)

Name: Mouse Anti-E2F2 Recombinant Antibody (CBFYE-0364)
SKU: V2LY-0425-LY1230
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-E2F2 Recombinant Antibody (CBFYE-0364) (V2LY-0425-LY1230-1) in WB

Western blot analysis for expression of SPINK1, phosphorylated and total MEK, phosphorylated and total ERK, CDK4, CDK6, Cyclin D1, phosphorylated and total Rb, E2F2, E2F1, E2F3, MCM3, PCNA and Cyclin A2 in MHCC97L cells with EV versus OE and PBS versus rhSPINK1, and in Huh7 cells with NTC versus SH2 versus SH3 and IgG versus nAb. ...View More

Man, K. F., Zhou, L., Yu, H., Lam, K. H., Cheng, W., Yu, J., ... & Ma, S. (2023). SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma. Nature communications, 14(1), 7863.

Mouse Anti-E2F2 Recombinant Antibody (CBFYE-0364) (V2LY-0425-LY1230-2) in IHC

Expression levels of E2F2 in gastric cancer tissues and normal gastric tissues were examined by immunohistochemical examination. ...View More

Chen, C., Zheng, Q., Pan, S., Chen, W., Huang, J., Cao, Y., ... & Jie, Z. (2021). The RNA-binding protein NELFE promotes gastric cancer growth and metastasis through E2F2. Frontiers in oncology, 11, 677111.

Mouse Anti-E2F2 Recombinant Antibody (CBFYE-0364) (V2LY-0425-LY1230-3) in WB

GSK5959 inhibited the protein levels of E2F2 and EZH2 in a dose-dependent manner after 5-day treatment. Error bars indicate mean±SD. Data were compared by independent t-test. Results were repeated at least three times. ...View More

Cheng, C. L. H., Tsang, F. H. C., Wei, L., Chen, M., Chin, D. W. C., Shen, J., ... & Wong, C. M. (2021). Bromodomain-containing protein BRPF1 is a therapeutic target for liver cancer. Communications Biology, 4(1), 888.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0364

Application

WB, IP, IF, IHC-P, ELISA

Immunogen

Full length of human E2F-2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

E2F transcription factor 2

Entrez Gene ID

Human	1870
Mouse	242705
Rat	684111

UniProt ID

Human	Q14209
Mouse	P56931
Rat	M0RDX6

Research Area

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Biological Process

Cell cycle Source: UniProtKB-KW
Intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
Lens fiber cell apoptotic process Source: Ensembl
Negative regulation of sprouting angiogenesis Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell cycle Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription initiation from RNA polymerase II promoter Source: ProtInc

Cellular Location

Nucleus

PTM

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Li, P., Xu, H., Yang, L., Zhan, M., Shi, Y., Zhang, C., ... & Wang, Z. (2022). E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1. Cell death & disease, 13(1), 1-12.

Lv, H., Yu, J., Zhang, H., Qian, X., Wang, Q., Lu, B., & Sun, Y. (2022). MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway. Bioengineered, 13(4), 8382-8395.

Xu, L., Wang, L., Shi, Y., Deng, Y., Oates, J. C., Kamen, D. L., ... & Tsao, B. P. (2022). Up‐Regulated Interleukin‐10 Induced by E2F Transcription Factor 2–MicroRNA‐17‐5p Circuitry in Extrafollicular Effector B Cells Contributes to Autoantibody Production in Systemic Lupus Erythematosus. Arthritis & Rheumatology, 74(3), 496-507.

Li, L., Zhang, Y., Wang, L., & Pan, J. (2021). ChIP-sequencing analysis of E2F transcription factor 2 reveals its role in various biological processes of rheumatoid arthritis synovial fibroblasts. BioScience Trends, 15(2), 132-134.

Li, L., Wang, S., Zhang, Y., & Pan, J. (2021). The E2F transcription factor 2: What do we know?. BioScience Trends.

Mamoor, S. (2021). Differential expression of E2F transcription factor 2 in human epithelial ovarian cancer.

Zhang, R., Wang, L., Pan, J. H., & Han, J. (2018). A critical role of E2F transcription factor 2 in proinflammatory cytokines-dependent proliferation and invasiveness of fibroblast-like synoviocytes in rheumatoid Arthritis. Scientific reports, 8(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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