Mouse Anti-EIF5A Recombinant Antibody (CBFYE-0079) (CBMAB-E0170-FY)

Name: Mouse Anti-EIF5A Recombinant Antibody (CBFYE-0079)
SKU: CBMAB-E0170-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EIF5A Recombinant Antibody (CBFYE-0079) (CBMAB-E0170-FY) in WB

Western blot measurement of protein expression of COL1A1, α-SMA, hypusinated eIF5A, and eIF5A in the vehicle and CPX-treated primary mouse CFs after vehicle or TGFβ treatment. Quantification of protein expression level was shown. ...View More

Subbaiah, K. C. V., Wu, J., Tang, W. H. W., & Yao, P. (2023). Ciclopirox inhibition of eIF5A hypusination attenuates fibroblast activation and cardiac fibrosis. Journal of Cardiovascular Development and Disease, 10(2), 52.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0079

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 9-124 mapping near the N-terminus of human eIF5A1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 1-154

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 5A

Introduction

EIF5A (Eukaryotic Translation Initiation Factor 5A) is a Protein Coding gene. Diseases associated with EIF5A include Human Immunodeficiency Virus Type 1 and Retinitis Pigmentosa 14. Among its related pathways are Metabolism of proteins and Translation Factors. Gene Ontology (GO) annotations related to this gene include protein N-terminus binding. An important paralog of this gene is EIF5AL1.

Entrez Gene ID

Human	1984
Mouse	276770
Rat	287444

UniProt ID

Human	P63241
Mouse	P63242
Rat	Q3T1J1

Alternative Names

Eukaryotic Translation Initiation Factor 5A; Rev-Binding Factor; EIF-5A-1; EIF-5A1; EIF-5A; EIF-4D

Research Area

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Biological Process

Cellular response to virus Source: UniProtKB
mRNA transport Source: UniProtKB-KW
Positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of translational elongation Source: UniProtKB
Positive regulation of translational termination Source: InterPro
Protein transport Source: UniProtKB-KW
Tumor necrosis factor-mediated signaling pathway Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Nucleus; Nuclear pore complex; Cytoplasm. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

PTM

Acetylated. Deacetylated by SIRT2.
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Barba-Aliaga, M., & Alepuz, P. (2022). Role of eIF5A in Mitochondrial Function. International Journal of Molecular Sciences, 23(3), 1284.

Li, H., Wu, B. K., Kanchwala, M., Cai, J., Wang, L., Xing, C., ... & Pan, D. (2022). YAP/TAZ drives cell proliferation and tumour growth via a polyamine–eIF5A hypusination–LSD1 axis. Nature cell biology, 24(3), 373-383.

Faundes, V., Jennings, M. D., Crilly, S., Legraie, S., Withers, S. E., Cuvertino, S., ... & Banka, S. (2021). Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine. Nature communications, 12(1), 1-13.

Park, M. H., Kar, R. K., Banka, S., Ziegler, A., & Chung, W. K. (2021). Post-translational formation of hypusine in eIF5A: Implications in human neurodevelopment. Amino acids, 1-15.

Bourourou, M., Gouix, E., Melis, N., Friard, J., Heurteaux, C., Tauc, M., & Blondeau, N. (2021). Inhibition of eIF5A hypusination pathway as a new pharmacological target for stroke therapy. Journal of Cerebral Blood Flow & Metabolism, 41(5), 1080-1090.

Cougnon, M., Carcy, R., Melis, N., Rubera, I., Duranton, C., Dumas, K., ... & Pisani, D. F. (2021). Inhibition of eIF5A hypusination reprogrammes metabolism and glucose handling in mouse kidney. Cell Death & Disease, 12(4), 1-14.

Coni, S., Serrao, S. M., Yurtsever, Z. N., Di Magno, L., Bordone, R., Bertani, C., ... & Canettieri, G. (2020). Blockade of EIF5A hypusination limits colorectal cancer growth by inhibiting MYC elongation. Cell death & disease, 11(12), 1-14.

Zhang, H., Alsaleh, G., Feltham, J., Sun, Y., Napolitano, G., Riffelmacher, T., ... & Simon, A. K. (2019). Polyamines control eIF5A hypusination, TFEB translation, and autophagy to reverse B cell senescence. Molecular cell, 76(1), 110-125.

Manjunath, H., Zhang, H., Rehfeld, F., Han, J., Chang, T. C., & Mendell, J. T. (2019). Suppression of ribosomal pausing by eIF5A is necessary to maintain the fidelity of start codon selection. Cell reports, 29(10), 3134-3146.

Turpaev, K. T. (2018). Translation factor eIF5A, modification with hypusine and role in regulation of gene expression. eIF5A as a target for pharmacological interventions. Biochemistry (Moscow), 83(8), 863-873.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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