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Mouse Anti-EPHB3 Recombinant Antibody (3F12) (CBMAB-A2659-LY)

The product is antibody recognizes EPHB3. The antibody 3F12 immunoassay techniques such as: WB, ELISA.
See all EPHB3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
3F12
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
EPHB3 (NP_004434, 899 a.a. ~ 997 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EPH Receptor B3
Introduction
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq]
Entrez Gene ID
Human2049
Mouse13845
UniProt ID
HumanP54753
MouseP54754
Alternative Names
ETK2; HEK2; TYRO6
Research Area
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Biological Process
Angiogenesis Source: UniProtKB
Axonal fasciculation Source: UniProtKB
Axon guidance Source: UniProtKB
Cell migration Source: UniProtKB
Central nervous system projection neuron axonogenesis Source: Ensembl
Corpus callosum development Source: UniProtKB
Dendritic spine development Source: UniProtKB
Dendritic spine morphogenesis Source: UniProtKB
Digestive tract morphogenesis Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of synapse assembly Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of axonogenesis Source: UniProtKB
Regulation of cell-cell adhesion Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Retinal ganglion cell axon guidance Source: Ensembl
Roof of mouth development Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB
Thymus development Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Urogenital system development Source: UniProtKB
Cellular Location
Cell membrane; Dendrite
Topology
Extracellular: 34-559
Helical: 560-580
Cytoplasmic: 581-998
PTM
Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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