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Mouse Anti-ERO1A Recombinant Antibody (CBFYE-0166) (CBMAB-E0394-FY)

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Published Data
CBMAB-E0394-FY-1
Mouse Anti-ERO1A Recombinant Antibody (CBFYE-0166) (CBMAB-E0394-FY) in WB
Representative Western blots and quantification of endoplasmic reticulum oxidase 1 (ERO1α) (n = 5-9), proliferating cell nuclear antigen (PCNA) (n = 4-10) and outer mitochondrial membrane 40 (TOM40) (n = 4-9) proteins in TF, Micro, Mito, and MAM. ...View More
Fernandes, T., Resende, R., Silva, D. F., Marques, A. P., Santos, A. E., Cardoso, S. M., ... & Pereira, C. F. (2021). Structural and functional alterations in mitochondria-associated membranes (Mams) and in mitochondria activate stress response mechanisms in an in vitro model of alzheimer's disease. Biomedicines, 9(8), 881.
CBMAB-E0394-FY-2
Mouse Anti-ERO1A Recombinant Antibody (CBFYE-0166) (CBMAB-E0394-FY) in WB
Images of Ero1α and P-eIF2α relative protein level measured by Western blot, normalized to β-actin. ...View More
Yao, W., Yang, X., Zhu, J., Gao, B., Liu, R., & Xu, L. (2017). Tang-Luo-Ning, a traditional Chinese medicine, inhibits endoplasmic reticulum stress-induced apoptosis of Schwann cells under high glucose environment. Evidence-Based Complementary and Alternative Medicine, 2017(1), 5193548.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYE-0166
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Recombinant human Ero1-Lα.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:5,000-1:10,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Endoplasmic Reticulum Oxidoreductase 1 Alpha
Introduction
ERO1A (Endoplasmic Reticulum Oxidoreductase 1 Alpha) is a Protein Coding gene. Diseases associated with ERO1A include Cholera. Among its related pathways are Peptide hormone metabolism and Detoxification of Reactive Oxygen Species. An important paralog of this gene is ERO1B.
Entrez Gene ID
Human30001
Mouse50527
Rat171562
Pig100192435
Dog490691
Horse100054748
Sheep101109883
UniProt ID
HumanQ96HE7
MouseQ8R180
RatQ8R4A1
PigB6CVD7
DogE2RNW5
HorseA0A3Q2HAI0
SheepW5Q834
Alternative Names
Endoplasmic Reticulum Oxidoreductase 1 Alpha; Endoplasmic Reticulum Oxidoreductase Alpha; Endoplasmic Oxidoreductin-1-Like Protein; Oxidoreductin-1-L-Alpha; ERO1-L-Alpha; ERO1-L; ERO1L; ERO1 (S. Cerevisiae)-Like
Research Area
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.
Biological Process
Animal organ senescence Source: Ensembl
Brown fat cell differentiation Source: Ensembl
Cell redox homeostasis Source: Ensembl
Cellular protein modification process Source: ProtInc
Cellular response to hypoxia Source: Ensembl
Chaperone cofactor-dependent protein refolding Source: UniProtKB
Endoplasmic reticulum unfolded protein response Source: Ensembl
Extracellular matrix organization Source: Ensembl
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
Peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: Ensembl
Protein folding Source: ProtInc
Protein folding in endoplasmic reticulum Source: GO_Central
Protein maturation by protein folding Source: Ensembl
Release of sequestered calcium ion into cytosol Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to oxidative stress Source: Ensembl
Response to temperature stimulus Source: ProtInc
Cellular Location
Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
PTM
N-glycosylated.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397 (By similarity). The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-131 (PubMed:23027870).

Liu, L., Li, S., Qu, Y., Wang, J., Fei, K., Wang, C., ... & Wang, J. (2022). Tumour ERO1A instigates T cell dysfunction by transmission of endoplasmic reticulum stress.

Luo, W., He, D., Zhang, J., Ma, Z., Chen, K., Fan, C., ... & Zhou, Z. (2021). Knockdown of PPARδ induces VEGFA-mediated angiogenesis via interaction with ERO1A in human colorectal cancer. Frontiers in oncology, 4184.

Huang, D., & Li, C. (2021). circ‑ACACA promotes proliferation, invasion, migration and glycolysis of cervical cancer cells by targeting the miR‑582‑5p/ERO1A signaling axis. Oncology Letters, 22(5), 1-12.

Zilli, F., Marques Ramos, P., Auf der Maur, P., Jehanno, C., Sethi, A., Coissieux, M. M., ... & Bentires‐Alj, M. (2021). The NFIB‐ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells. EMBO molecular medicine, 13(4), e13162.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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