Mouse Anti-ERRFI1 Recombinant Antibody (2B9) (V2LY-0825-LY745)

Name: Mouse Anti-ERRFI1 Recombinant Antibody (2B9)
SKU: V2LY-0825-LY745
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B9

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 156-280 mapping within an internal region of human Mig-6.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ERBB receptor feedback inhibitor 1

Entrez Gene ID

54206

UniProt ID

Q9UJM3

Research Area

Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development (By similarity).

Biological Process

Cellular hyperosmotic response Source: Ensembl
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Cellular response to platelet-derived growth factor stimulus Source: Ensembl
Lung alveolus development Source: UniProtKB
Lung epithelium development Source: UniProtKB
Lung vasculature development Source: UniProtKB
Negative regulation of cardiac muscle hypertrophy in response to stress Source: Ensembl
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Negative regulation of epidermal growth factor receptor signaling pathway Source: GO_Central
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of interleukin-1 beta production Source: Ensembl
Negative regulation of protein autophosphorylation Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: Ensembl
Regulation of keratinocyte differentiation Source: UniProtKB
Regulation of type B pancreatic cell proliferation Source: Ensembl
Skin morphogenesis Source: UniProtKB

Cellular Location

Nucleus; Cell membrane; Cytoplasm. Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes (By similarity).

Wu, K., Chen, X., & Ma, S. (2022). EP05. 01-031 Lysimachia Capillipes Capilliposide C Enhances the Radiosensitivity of Lung Cancer by Promoting ERRFI1 via Inhibiting Phosphorylation of STAT3. Journal of Thoracic Oncology, 17(9), S281.

Xu, D., & Li, C. (2021). Gene 33/Mig6/ERRFI1, an Adapter Protein with Complex Functions in Cell Biology and Human Diseases. Cells, 10(7), 1574.

Yu, Y., Chen, Q., Zhang, X., Yang, J., Lin, K., Ji, C., ... & Miao, L. (2020). Long noncoding RNA ANRIL promotes the malignant progression of cholangiocarcinoma by epigenetically repressing ERRFI1 expression. Cancer science, 111(7), 2297-2309.

Ma, P., Zhang, C., Huo, P., Li, Y., & Yang, H. (2020). A novel role of the miR‐152‐3p/ERRFI1/STAT3 pathway modulates the apoptosis and inflammatory response after acute kidney injury. Journal of biochemical and molecular toxicology, 34(9), e22540.

Pan, R., Cai, W., Sun, J., Yu, C., Li, P., & Zheng, M. (2020). Inhibition of KHSRP sensitizes colorectal cancer to 5‐fluoruracil through miR‐501‐5p‐mediated ERRFI1 mRNA degradation. Journal of Cellular Physiology, 235(2), 1576-1587.

Wang, M., Zhao, Y., Yu, Z. Y., Zhang, R. D., Li, S. A., Zhang, P., ... & Sun, H. W. (2020). Glioma exosomal microRNA-148a-3p promotes tumor angiogenesis through activating the EGFR/MAPK signaling pathway via inhibiting ERRFI1. Cancer cell international, 20(1), 1-16.

Wang, W., Zheng, Y., Wang, M., Yan, M., Jiang, J., & Li, Z. (2019). Exosomes derived miR-126 attenuates oxidative stress and apoptosis from ischemia and reperfusion injury by targeting ERRFI1. Gene, 690, 75-80.

Guo, Y., Tian, L., Liu, X., He, Y., Chang, S., & Shen, Y. (2019). ERRFI1 inhibits proliferation and inflammation of nucleus pulposus and is negatively regulated by miR-2355-5p in intervertebral disc degeneration. Spine, 44(15), E873-E881.

Migliore, C., Morando, E., Ghiso, E., Anastasi, S., Leoni, V. P., Apicella, M., ... & Giordano, S. (2018). miR‐205 mediates adaptive resistance to MET inhibition via ERRFI 1 targeting and raised EGFR signaling. EMBO molecular medicine, 10(9), e8746.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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