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Mouse Anti-FBL Recombinant Antibody (CBXF-1726) (CBMAB-F3261-CQ)

This product is a mouse antibody that recognizes FBL. The antibody CBXF-1726 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all FBL antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXF-1726
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Fibrillarin
Introduction
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Entrez Gene ID
Human2091
Mouse14113
Rat292747
UniProt ID
HumanP22087
MouseP35550
RatP22509
Alternative Names
Fibrillarin; 34 KDa Nucleolar Scleroderma Antigen; Histone-Glutamine Methyltransferase; FLRN; RNA, U3 Small Nucleolar Interacting Protein 1; RRNA 2-O-Methyltransferase Fibrillarin; 34-KD Nucleolar Scleroderma Antigen; EC 2.1.1.37;
Research Area
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898).

Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930).

Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity).

Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity).

Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898).

U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898).

Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930).
Biological Process
Box C/D RNA 3'-end processing Source: GO_Central
Histone glutamine methylation Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
rRNA methylation Source: UniProtKB
rRNA processing Source: ProtInc
SnoRNA localization Source: UniProtKB
Cellular Location
Nucleolus; Nucleoplasm. Fibrillar region of the nucleolus.
PTM
Ubiquitinated. Ubiquitination leads to proteasomal degradation (PubMed:19208757). Deubiquitinated by USP36 (PubMed:19208757).
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).By similarity
Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation (PubMed:30540930). Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930).

Luo, W., Lin, S., Huang, Y., Zhu, K., Zhang, F., Lin, J., ... & Liu, C. (2022). Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma. Bioengineering, 9(8), 396.

Nguyen Van Long, F., Lardy-Cleaud, A., Carène, D., Rossoni, C., Catez, F., Rollet, P., ... & Marcel, V. (2022). Low level of Fibrillarin, a ribosome biogenesis factor, is a new independent marker of poor outcome in breast cancer. BMC cancer, 22(1), 1-12.

Zhang, J., Yang, G., Li, Q., & Xie, F. (2021). Increased fibrillarin expression is associated with tumor progression and an unfavorable prognosis in hepatocellular carcinoma. Oncology Letters, 21(2), 1-1.

Wang, T., & Na, J. (2021). Fibrillarin-GFP facilitates the identification of meiotic competent oocytes. Frontiers in cell and developmental biology, 9, 648331.

Shubina, M. Y., Arifulin, E. A., Sorokin, D. V., Sosina, M. A., Tikhomirova, M. A., Serebryakova, M. V., ... & Sheval, E. V. (2020). The GAR domain integrates functions that are necessary for the proper localization of fibrillarin (FBL) inside eukaryotic cells. PeerJ, 8, e9029.

El Hassouni, B., Sarkisjan, D., Vos, J. C., Giovannetti, E., & Peters, G. J. (2019). Targeting the ribosome biogenesis key molecule fibrillarin to avoid chemoresistance. Current medicinal chemistry, 26(33), 6020-6032.

Iyer-Bierhoff, A., Krogh, N., Tessarz, P., Ruppert, T., Nielsen, H., & Grummt, I. (2018). SIRT7-dependent deacetylation of fibrillarin controls histone H2A methylation and rRNA synthesis during the cell cycle. Cell reports, 25(11), 2946-2954.

Shubina, M. Y., Musinova, Y. R., & Sheval, E. V. (2018). Proliferation, cancer, and aging—novel functions of the nucleolar methyltransferase fibrillarin?. Cell Biology International, 42(11), 1463-1466.

Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., ... & Jamen, F. (2018). Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina. Developmental biology, 437(1), 1-16.

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For research use only. Not intended for any clinical use.

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