Mouse Anti-FBP2 Recombinant Antibody (CAP1032) (V2LY-0925-LY374)

Name: Mouse Anti-FBP2 Recombinant Antibody (CAP1032)
SKU: V2LY-0925-LY374
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FBP2 Recombinant Antibody (CAP1032) (V2LY-0925-LY374) in IF

Co2+ stimulates Fbp2 association with mitochondria but it does not alter the nuclear accumulation of the protein. (A) Fbp-mitochondria colocalization is significantly increased in Co2+-treated neurons both in the presence and in the absence of Fbp inhibitor (iFbp). In the presence of Co2+, the amount of mitochondria-bound Fbp is as high as after the LTP induction. The iFbp-treated neurons show reduced colocalization compared to control cells. (B) Co2+ treatment does not change the nuclear accumulation of Fbp in neurons. ...View More

Duda, P., Budziak, B., & Rakus, D. (2021). Cobalt regulates activation of Camk2α in neurons by influencing fructose 1, 6-bisphosphatase 2 quaternary structure and subcellular localization. International journal of molecular sciences, 22(9), 4800.

Mouse Anti-FBP2 Recombinant Antibody (CAP1032) (V2LY-0925-LY374) in IF

(A) Fluorescence signals associated with phosphorylated forms of Camk2α, AMPAR (Gria1) and Gsk3β are not affected by Fbp2 silencing or inhibition/tetramerization in neurons in which LTP was not induced. (B) Induction of LTP stimulates phosphorylation of Camk2α, AMPAR and Gsk3β (upper row) but manipulation with Fbp2 amount and structure strongly reduces the phosphorylation. ...View More

Duda, P., Wójtowicz, T., Janczara, J., Krowarsch, D., Czyrek, A., Gizak, A., & Rakus, D. (2020). Fructose 1, 6-bisphosphatase 2 plays a crucial role in the induction and maintenance of long-term potentiation. Cells, 9(6), 1375.

Mouse Anti-FBP2 Recombinant Antibody (CAP1032) (V2LY-0925-LY374) in IF

NCM reduces the level of Fbp2-protein-related fluorescence in astrocytes. The graph presents the quantification of the Fbp-related immunofluorescent signal. ...View More

Hajka, D., Duda, P., Wójcicka, O., Drulis-Fajdasz, D., Rakus, D., & Gizak, A. (2020). Expression of FBP2, a newly discovered constituent of memory formation mechanisms, is regulated by astrocyte-neuron crosstalk. International journal of molecular sciences, 21(18), 6903.

Mouse Anti-FBP2 Recombinant Antibody (CAP1032) (V2LY-0925-LY374) in IF

Fbp2 is the main fructose bisphosphatase isoform in murine and human cancer cells. ...View More

Duda, P., Janczara, J., McCubrey, J. A., Gizak, A., & Rakus, D. (2020). The reverse Warburg effect is associated with Fbp2-dependent Hif1α regulation in cancer cells stimulated by fibroblasts. Cells, 9(1), 205.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CAP1032

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 287-317 near the C-terminus of human muscle FBPase.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Horse

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fructose-Bisphosphatase 2

Entrez Gene ID

Human	8789
Mouse	14120
Rat	114508

UniProt ID

Human	O00757
Mouse	P70695
Rat	Q9Z1N1

Research Area

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.

Biological Process

Fructose 1,6-bisphosphate metabolic process Source: GO_Central
Fructose 6-phosphate metabolic process Source: GO_Central
Fructose metabolic process Source: GO_Central
Gluconeogenesis Source: GO_Central
Sucrose biosynthetic process Source: GO_Central

Cellular Location

Cytoplasm; Nucleus; Cell junction; Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca2+ concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca2+ concentration.

Bakshi, I., Suryana, E., Small, L., Quek, L. E., Brandon, A. E., Turner, N., & Cooney, G. J. (2018). Fructose bisphosphatase 2 overexpression increases glucose uptake in skeletal muscle. Journal of Endocrinology, 237(2), 101-111.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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