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Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319)

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Published Data
V2LY-0925-LY1319-1
Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319) in IF
Representative immunofluorescence images showing GPR120 localization in WT and AMPKα2−/− VSMCs, before and after a 1-h DHA treatment. Scale bar, 25 μm. ...View More
Bódis, K., Kahl, S., Simon, M. C., Zhou, Z., Sell, H., Knebel, B., ... & Roden, M. (2018). Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutrition & diabetes, 8(1), 49.
V2LY-0925-LY1319-2
Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319) in Immunoblot
A, B IP assays showed endogenous GPR120 and SUMOylated GPR120 in WT and AMPKα2−/− VSMCs by western blotting of whole cell extracts, prepared in the presence of isopeptidase inhibitors. ...View More
Bódis, K., Kahl, S., Simon, M. C., Zhou, Z., Sell, H., Knebel, B., ... & Roden, M. (2018). Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus. Nutrition & diabetes, 8(1), 49.
V2LY-0925-LY1319-3
Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319) in IF
Immunofluorescence analysis examined the expression of GPR120 and IP3R in STC-1 cells. The scale bar represents 20 μm. Green, GPR120; Red, IP3R. ...View More
Chen, X., Nie, X., Wang, H., Yan, S., & Zhang, Y. (2022). Stearic acid induces CCK and GLP-1 upregulation via GPR120/PLC-β, leading to reduced appetite in Hu sheep fed with rice straw. Frontiers in Veterinary Science, 9, 948074.
V2LY-0925-LY1319-4
Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319) in ICC
The ICC analysis of FFAR2 (green pseudocolor) and FFAR4 (red) expression on separated cell cultures of ECs, Ast., hOPCs, and mature OLs. ...View More
Piatek, P., Lewkowicz, N., Michlewska, S., Wieczorek, M., Bonikowski, R., Parchem, K., ... & Namiecinska, M. (2022). Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood-brain barrier. Frontiers in immunology, 13, 932383.
V2LY-0925-LY1319-5
Mouse Anti-FFAR4 Recombinant Antibody (EG1368) (V2LY-0925-LY1319) in IF
GPR120 and NLRP3 co-localization was observed in immunofluorescence (scale bar, 20 μm). ...View More
Fan, G., Li, Y., Chen, J., Zong, Y., & Yang, X. (2021). DHA/AA alleviates LPS-induced Kupffer cells pyroptosis via GPR120 interaction with NLRP3 to inhibit inflammasome complexes assembly. Cell death & disease, 12(1), 73.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
EG1368
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 78-232 mapping at the C-terminus of human GPR120.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
free fatty acid receptor 4
Entrez Gene ID
Human338557
Mouse107221
Rat294075
UniProt ID
HumanQ5NUL3
MouseQ7TMA4
RatQ2AC31
Biological Process
Brown fat cell differentiation Source: UniProtKB
Ghrelin secretion Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Hormone secretion Source: GO_Central
Inflammatory response Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of interleukin-1 beta production Source: UniProtKB
Negative regulation of somatostatin secretion Source: UniProtKB
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Positive regulation of brown fat cell differentiation Source: Ensembl
Positive regulation of cAMP-mediated signaling Source: UniProtKB
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of glucagon secretion Source: UniProtKB
Positive regulation of osteoblast differentiation Source: UniProtKB
Regulation of glucose transmembrane transport Source: UniProtKB
White fat cell differentiation Source: UniProtKB
Cellular Location
Isoform 1: Cell membrane; Lysosome membrane; Endosome membrane. Sorted to late endosome/lysosome compartments upon internalization.
Isoform 2: Cell membrane; Cilium membrane; Lysosome membrane; Endosome membrane. Sorted to late endosome/lysosome compartments upon internalization (PubMed:22282525). Specifically localizes to the primary cilium of undifferentiated adipocytes. Ciliary trafficking is TULP3-dependent. As the cilium is lost during adipogenesis, moves to the plasma membrane (Probable).
Topology
Extracellular: 1-45
Helical: 46-66
Cytoplasmic: 67-77
Helical: 78-98
Extracellular: 99-112
Helical: 113-133
Cytoplasmic: 134-156
Helical: 157-177
Extracellular: 178-204
Helical: 205-225
Cytoplasmic: 226-268
Helical: 269-289
Extracellular: 290-295
Helical: 296-316
Cytoplasmic: 317-361
PTM
Phosphorylated at two clusters of Ser and Thr residues located in the intracellular C-terminus, a prerequisite for FFAR4 internalization via an ARRB2-dependent pathway.

Zhang, N., Murphy, K. A., Harsch, B., Zhang, M., Gyberg, D. J., Wagner, B. M., ... & O'Connell, T. (2022). Free fatty acid receptor 4 (FFAR4) regulates cardiac oxylipin balance to promote inflammation resolution in a model of heart failure preserved ejection fraction secondary to metabolic syndrome. bioRxiv.

Yang, L., Wang, B., Guo, F., Huang, R., Liang, Y., Li, L., ... & Ma, L. (2022). FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury. Signal Transduction and Targeted Therapy, 7(1), 1-15.

Stuttgen, G. M., & Sahoo, D. (2021). FFAR4: a new player in cardiometabolic disease?. Endocrinology, 162(8).

Kiepura, A., Stachyra, K., & Olszanecki, R. (2021). Anti-atherosclerotic potential of free fatty acid receptor 4 (FFAR4). Biomedicines, 9(5), 467.

Mishra, S., Rajput, M. S., Rathore, D., & Dahima, R. (2021). Ligand and structure-based computational designing of multi-target molecules directing FFAR-1, FFAR-4 and PPAR-G as modulators of insulin receptor activity. Journal of Biomolecular Structure and Dynamics, 1-15.

Carullo, G., Mazzotta, S., Vega-Holm, M., Iglesias-Guerra, F., Vega-Pérez, J. M., Aiello, F., & Brizzi, A. (2021). GPR120/FFAR4 pharmacology: focus on agonists in type 2 diabetes mellitus drug discovery. Journal of Medicinal Chemistry, 64(8), 4312-4332.

Husted, A. S., Ekberg, J. H., Tripp, E., Nissen, T. A., Meijnikman, S., O'Brien, S. L., ... & Schwartz, T. W. (2020). Autocrine negative feedback regulation of lipolysis through sensing of NEFAs by FFAR4/GPR120 in WAT. Molecular metabolism, 42, 101103.

Tsuchiya, Y., Hayashi, M., Nagamatsu, K., Ono, T., Kamakura, M., Iwata, T., & Nakashima, T. (2020). The key royal jelly component 10-hydroxy-2-decenoic acid protects against bone loss by inhibiting NF-κB signaling downstream of FFAR4. Journal of Biological Chemistry, 295(34), 12224-12232.

Costanzo, A., Liu, D., Nowson, C., Duesing, K., Archer, N., Bowe, S., & Keast, R. (2019). A low-fat diet up-regulates expression of fatty acid taste receptor gene FFAR4 in fungiform papillae in humans: A co-twin randomised controlled trial. British journal of nutrition, 122(11), 1212-1220.

Chu, X., Zhou, Q., Xu, Y., Jiang, J., Li, Q., Zhou, Q., ... & Zhang, Y. (2019). Aberrant fatty acid profile and FFAR4 signaling confer endocrine resistance in breast cancer. Journal of Experimental & Clinical Cancer Research, 38(1), 1-18.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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