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Mouse Anti-FHOD1 Recombinant Antibody (3F7) (CBMAB-A2986-LY)

The product is antibody recognizes FHOD1. The antibody 3F7 immunoassay techniques such as: WB, ELISA.
See all FHOD1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F7
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
FHOD1 (NP_037373.1, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
formin homology 2 domain containing 1
Introduction
This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. [provided by RefSeq]
Entrez Gene ID
29109
UniProt ID
Q9Y613
Alternative Names
FHOS
Function
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Biological Process
Actin filament network formation Source: GO_Central
Cortical actin cytoskeleton organization Source: GO_Central
Establishment of centrosome localization Source: Ensembl
Nuclear migration Source: Ensembl
Positive regulation of stress fiber assembly Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of stress fiber assembly Source: CAFA
Cellular Location
Cytoplasm; Cytoskeleton; Bleb. Predominantly cytoplasmic.
PTM
Phosphorylated by ROCK1.

Hałas-Wiśniewska, M., Izdebska, M., Zielińska, W., & Grzanka, A. (2021). Downregulation of FHOD1 Inhibits Metastatic Potential in A549 Cells. Cancer Management and Research, 13, 91.

Zuidscherwoude, M., Haining, E. J., Simms, V. A., Watson, S., Grygielska, B., Hardy, A. T., ... & Thomas, S. G. (2021). Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function. Platelets, 32(8), 1051-1062.

Jiang, C., Yuan, B., Hang, B., Mao, J. H., Zou, X., & Wang, P. (2021). FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncology letters, 22(4), 1-11.

Mansuri, N., Heuser, V. D., Birkman, E. M., Lintunen, M., Ålgars, A., Sundström, J., ... & Lehtinen, L. (2021). FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes. Gastric Cancer, 24(6), 1254-1263.

Lim, S. M., Cruz, V. E., Antoku, S., Gundersen, G. G., & Schwartz, T. U. (2021). Structures of FHOD1-Nesprin1/2 complexes reveal alternate binding modes for the FH3 domain of formins. Structure, 29(6), 540-552.

Sanematsu, F., Kanai, A., Ushijima, T., Shiraishi, A., Abe, T., Kage, Y., ... & Takeya, R. (2019). Fhod1, an actin‐organizing formin family protein, is dispensable for cardiac development and function in mice. Cytoskeleton, 76(2), 219-229.

Shi, X., Zhao, S., Cai, J., Wong, G., & Jiu, Y. (2019). Active FHOD1 promotes the formation of functional actin stress fibers. Biochemical Journal, 476(20), 2953-2963.

Heuser, V. D., Mansuri, N., Mogg, J., Kurki, S., Repo, H., Kronqvist, P., ... & Gardberg, M. (2018). Formin proteins FHOD1 and INF2 in triple-negative breast cancer: association with basal markers and functional activities. Breast Cancer: Basic and Clinical Research, 12, 1178223418792247.

Refai, O., Smit, R. B., Votra, S., Pruyne, D., & Mains, P. E. (2018). Tissue-specific functions of fem-2/PP2c phosphatase and fhod-1/formin during Caenorhabditis elegans embryonic morphogenesis. G3: Genes, Genomes, Genetics, 8(7), 2277-2290.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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