Mouse Anti-FHOD1 Recombinant Antibody (CBXF-0681) (V2LY-0925-LY1954)

Name: Mouse Anti-FHOD1 Recombinant Antibody (CBXF-0681)
SKU: V2LY-0925-LY1954
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0681

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 34-110 mapping near the N-terminus of human FHOD1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

formin homology 2 domain containing 1

Entrez Gene ID

29109

UniProt ID

Q9Y613

Function

Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.

Biological Process

Actin filament network formation Source: GO_Central
Cortical actin cytoskeleton organization Source: GO_Central
Establishment of centrosome localization Source: Ensembl
Nuclear migration Source: Ensembl
Positive regulation of stress fiber assembly Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of stress fiber assembly Source: CAFA

Cellular Location

Cytoplasm; Cytoskeleton; Bleb. Predominantly cytoplasmic.

PTM

Phosphorylated by ROCK1.

Hałas-Wiśniewska, M., Izdebska, M., Zielińska, W., & Grzanka, A. (2021). Downregulation of FHOD1 Inhibits Metastatic Potential in A549 Cells. Cancer Management and Research, 13, 91.

Zuidscherwoude, M., Haining, E. J., Simms, V. A., Watson, S., Grygielska, B., Hardy, A. T., ... & Thomas, S. G. (2021). Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function. Platelets, 32(8), 1051-1062.

Jiang, C., Yuan, B., Hang, B., Mao, J. H., Zou, X., & Wang, P. (2021). FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncology letters, 22(4), 1-11.

Mansuri, N., Heuser, V. D., Birkman, E. M., Lintunen, M., Ålgars, A., Sundström, J., ... & Lehtinen, L. (2021). FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes. Gastric Cancer, 24(6), 1254-1263.

Lim, S. M., Cruz, V. E., Antoku, S., Gundersen, G. G., & Schwartz, T. U. (2021). Structures of FHOD1-Nesprin1/2 complexes reveal alternate binding modes for the FH3 domain of formins. Structure, 29(6), 540-552.

Sanematsu, F., Kanai, A., Ushijima, T., Shiraishi, A., Abe, T., Kage, Y., ... & Takeya, R. (2019). Fhod1, an actin‐organizing formin family protein, is dispensable for cardiac development and function in mice. Cytoskeleton, 76(2), 219-229.

Shi, X., Zhao, S., Cai, J., Wong, G., & Jiu, Y. (2019). Active FHOD1 promotes the formation of functional actin stress fibers. Biochemical Journal, 476(20), 2953-2963.

Heuser, V. D., Mansuri, N., Mogg, J., Kurki, S., Repo, H., Kronqvist, P., ... & Gardberg, M. (2018). Formin proteins FHOD1 and INF2 in triple-negative breast cancer: association with basal markers and functional activities. Breast Cancer: Basic and Clinical Research, 12, 1178223418792247.

Refai, O., Smit, R. B., Votra, S., Pruyne, D., & Mains, P. E. (2018). Tissue-specific functions of fem-2/PP2c phosphatase and fhod-1/formin during Caenorhabditis elegans embryonic morphogenesis. G3: Genes, Genomes, Genetics, 8(7), 2277-2290.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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