Mouse Anti-FIGN Recombinant Antibody (CBXF-3032) (V2LY-0925-LY1973)

Name: Mouse Anti-FIGN Recombinant Antibody (CBXF-3032)
SKU: V2LY-0925-LY1973
Rating: 5 (1 reviews)

See all FIGN antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-3032

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 75-220 mapping near the N-terminus of human fidgetin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fidgetin, Microtubule Severing Factor

Entrez Gene ID

Human	55137
Mouse	60344
Rat	295649

UniProt ID

Human	Q5HY92
Mouse	Q9ERZ6
Rat	D3ZYS4

Function

ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, that may lead to the suppression of microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cytoplasmic microtubule organization Source: GO_Central

Cellular Location

Centrosome; Nucleus matrix. Localizes to centrosomes throughout mitosis and to the spindle midzone during telophase.

Li, J., Wu, F., Cheng, L., Zhang, J., Cha, C., Chen, L., ... & Guo, G. (2020). A nuclear localization signal is required for the nuclear translocation of Fign and its microtubule‑severing function. Molecular medicine reports, 21(6), 2367-2374.

Matamoros, A. J., Tom, V. J., Wu, D., Rao, Y., Sharp, D. J., & Baas, P. W. (2019). Knockdown of fidgetin improves regeneration of injured axons by a microtubule-based mechanism. Journal of Neuroscience, 39(11), 2011-2024.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-FIGN Recombinant Antibody (CBXF-3032)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-FIGN Recombinant Antibody (CBXF-1747) (CAT#: V2LY-0925-LY1972)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry