Mouse Anti-FMN2 Recombinant Antibody (1C2) (CBMAB-A3053-LY)

Name: Mouse Anti-FMN2 Recombinant Antibody (1C2)
SKU: CBMAB-A3053-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1C2

Antibody Isotype

IgG2b, κ

Application

WB, ELISA

Basic Information

Immunogen

FMN2 (AAH14364.2, 144 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Formin 2

Introduction

Formin homology (FH) domain proteins (e.g., FMN; MIM 136535) play a role in cytoskeletal organization and/or establishment of cell polarity.[supplied by OMIM

Entrez Gene ID

56776

UniProt ID

Q9NZ56

Function

Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization (PubMed:22330775, PubMed:21730168).

Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2 (PubMed:22330775, PubMed:21730168).

Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (By similarity).

Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity).

Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest (PubMed:23375502).

Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480).

Protects cells against apoptosis by protecting CDKN1A against degradation (PubMed:23375502).

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to hypoxia Source: UniProtKB
Establishment of meiotic spindle localization Source: BHF-UCL
Formin-nucleated actin cable assembly Source: UniProtKB
Homologous chromosome movement towards spindle pole in meiosis I anaphase Source: BHF-UCL
Intracellular signal transduction Source: InterPro
Intracellular transport Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of protein catabolic process Source: UniProtKB
Oogenesis Source: BHF-UCL
Polar body extrusion after meiotic divisions Source: BHF-UCL
Positive regulation of double-strand break repair Source: UniProtKB
Protein transport Source: UniProtKB-KW
Vesicle-mediated transport Source: UniProtKB

Cellular Location

Nucleus; Nucleolus; Cell membrane; Cytoskeleton; Cytosol; Perinuclear region; Cytoplasmic vesicle membrane; Cell cortex. Colocalizes with the actin cytoskeleton (PubMed:20082305). Recruited to the membranes via its interaction with SPIRE1 (By similarity). Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion (By similarity). Accumulates in the nucleus following DNA damage (PubMed:26287480).

Involvement in disease

Mental retardation, autosomal recessive 47 (MRT47):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT47 patients show delayed development, with cognition and speech more affected than motor skills.

More Infomation

References

Gorukmez, O., Gorukmez, O., & Ekici, A. (2021). A novel nonsense FMN2 mutation in nonsyndromic autosomal recessive intellectual disability syndrome. Fetal and Pediatric Pathology, 40(6), 702-706.

Ghate, K., Mutalik, S. P., Sthanam, L. K., Sen, S., & Ghose, A. (2020). Fmn2 regulates growth cone motility by mediating a molecular clutch to generate traction forces. Neuroscience, 448, 160-171.

CS, J. (2020). The role of Formin-2 in the Development of Rectinotectal connectivity in Danio rerio (Doctoral dissertation).

KUNDU, T. (2019). Role of Formin-2 in actin-microtubule coordination during axonal pathfinding and it's characterization in axonal branching (Doctoral dissertation, Dept. of Biology).

Lian, G., Chenn, A., Ekuta, V., Kanaujia, S., & Sheen, V. (2019). Formin 2 regulates lysosomal degradation of Wnt-associated β-catenin in neural progenitors. Cerebral Cortex, 29(5), 1938-1952.

Prakash, M. (2019). Evaluation of the role of Formin-2 in the devleopment and function of the posterior lateral line in zebrafish (Doctoral dissertation).

Li, D. J., Feng, Z. C., Li, X. R., & Hu, G. (2018). Involvement of methylation-associated silencing of formin 2 in colorectal carcinogenesis. World journal of gastroenterology, 24(44), 5013.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Mouse Anti-FMN2 Recombinant Antibody (CBXF-3098)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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