Mouse Anti-FMO3 (AA 1-291) Recombinant Antibody (3H1) (CBMAB-F2290-CQ)

Name: Mouse Anti-FMO3 (AA 1-291) Recombinant Antibody (3H1)
SKU: CBMAB-F2290-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3H1

Application

IHC-P, WB

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-291

More Infomation

Target

Full Name

Flavin Containing Monooxygenase 3

Introduction

Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.

Entrez Gene ID

2328

UniProt ID

P31513

Alternative Names

Flavin Containing Monooxygenase 3; Trimethylamine Monooxygenase; Dimethylaniline Oxidase 3; EC 1.14.13.8; FMO Form 2; Dimethylaniline Monooxygenase [N-Oxide-Forming] 3; Hepatic Flavin-Containing Monooxygenase-3; Hepatic Flavin-Containing Monooxygenase 3;

Function

Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds (PubMed:10759686, PubMed:30381441).

Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite (PubMed:9776311).

TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation (PubMed:29981269).

Cellular Location

Microsome membrane; Endoplasmic reticulum membrane

Involvement in disease

Trimethylaminuria (TMAU):
Inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Affected individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine.

Topology

Helical: 510-530

Shorudi Dadi, I., Saravani, R., Khalili, T., Majidpour, M., Sarhadi, M., Mirinejad, S., ... & Alidadi, A. (2022). Coding Variants of the FMO3 Gene Are Associated with the Risk of Chronic Kidney Disease: A Case-Control Study. Reports of Biochemistry and Molecular Biology, 11(3), 430-439.

Indra, R., Pompach, P., Vavrová, K., Jáklová, K., Heger, Z., Adam, V., ... & Stiborová, M. (2020). Cytochrome P450 and flavin-containing monooxygenase enzymes are responsible for differential oxidation of the anti-thyroid-cancer drug vandetanib by human and rat hepatic microsomal systems. Environmental Toxicology and Pharmacology, 74, 103310.

Bryant, T. S., Duggal, P., Yu, B., Morrison, A. C., Shafi, T., Ehret, G., ... & Tin, A. (2019). Association of FMO3 variants with blood pressure in the atherosclerosis risk in communities study. International journal of hypertension, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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