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Mouse Anti-FZD5 Recombinant Antibody (6A3) (CBMAB-A3255-LY)

The product is antibody recognizes FZD5. The antibody 6A3 immunoassay techniques such as: WB, ELISA.
See all FZD5 antibodies
Published Data
Fig.1 Fluorescence confocal microscopy was used to detect localization of FZD5 in 2H11 cells.
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Fig.2 Western blot analysis on samples 2H11 cells showing the levels of FZD5.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
6A3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
FZD5 (ENSP00000354607, 72 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
frizzled class receptor 5
Introduction
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq]
Entrez Gene ID
7855
UniProt ID
Q13467
Alternative Names
C2orf31; DKFZp434E2135; HFZ5; MGC129692
Function
Receptor for Wnt proteins (PubMed:9054360, PubMed:10097073, PubMed:20530549).

Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (By similarity).

In neurons, activation of WNT7A promotes formation of synapses (PubMed:20530549).

Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity).
Biological Process
Angiogenesis Source: Ensembl
Anterior/posterior axis specification, embryo Source: BHF-UCL
Apoptotic process involved in morphogenesis Source: Ensembl
Branching involved in labyrinthine layer morphogenesis Source: Ensembl
Canonical Wnt signaling pathway Source: BHF-UCL
Cell maturation Source: Ensembl
Cellular response to molecule of bacterial origin Source: BHF-UCL
Chorionic trophoblast cell differentiation Source: Ensembl
Embryonic axis specification Source: BHF-UCL
Embryonic camera-type eye morphogenesis Source: Ensembl
Labyrinthine layer blood vessel development Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Neuron differentiation Source: UniProtKB
Positive regulation of interferon-gamma production Source: BHF-UCL
Positive regulation of interleukin-1 beta production Source: ARUK-UCL
Positive regulation of JUN kinase activity Source: Ensembl
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Positive regulation of T cell cytokine production Source: CACAO
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of tumor necrosis factor production Source: ARUK-UCL
Post-embryonic camera-type eye development Source: Ensembl
Regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
Regulation of bicellular tight junction assembly Source: Ensembl
Regulation of chorionic trophoblast cell proliferation Source: Ensembl
Spemann organizer formation Source: BHF-UCL
Synapse assembly Source: ParkinsonsUK-UCL
Syncytiotrophoblast cell differentiation involved in labyrinthine layer development Source: Ensembl
T cell differentiation in thymus Source: Ensembl
Cellular Location
Golgi apparatus membrane; Cell membrane; Synapse; Perikaryon; Dendrite; Axon. Localized at the plasma membrane and also found at the Golgi.
Topology
Extracellular: 27-238
Helical: 239-259
Cytoplasmic: 260-270
Helical: 271-291
Extracellular: 292-315
Helical: 316-336
Cytoplasmic: 337-358
Helical: 359-379
Extracellular: 380-402
Helical: 403-423
Cytoplasmic: 424-449
Helical: 450-470
Extracellular: 471-500
Helical: 501-521
Cytoplasmic: 522-585
PTM
Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.

Cao, G., Long, K., Qiu, Y., Ma, Y., Qin, H., Huo, D., ... & Hou, C. (2023). Inducible positive amplification regulation coupled with the double-strand specific nuclease for FzD5 mRNA assay. Microchemical Journal, 184, 108179.

Holt, R., Goudie, D., Verde, A. D., Gardham, A., Ramond, F., Putoux, A., ... & Ragge, N. K. (2022). Individuals with heterozygous variants in the Wnt-signalling pathway gene FZD5 delineate a phenotype characterized by isolated coloboma and variable expressivity. Ophthalmic Genetics, 43(6), 809-816.

Peng, N., Zhang, Z., Wang, Y., Yang, M., Fan, J., Wang, Q., ... & Li, W. (2021). Down‐regulated LINC00115 inhibits prostate cancer cell proliferation and invasion via targeting miR‐212‐5p/FZD5/Wnt/β‐catenin axis. Journal of Cellular and Molecular Medicine, 25(22), 10627-10637.

Aubert-Mucca, M., Pernin-Grandjean, J., Marchasson, S., Gaston, V., Habib, C., Meunier, I., ... & Plaisancié, J. (2021). Confirmation of FZD5 implication in a cohort of 50 patients with ocular coloboma. European Journal of Human Genetics, 29(1), 131-140.

Liu, X., Li, Y., Chen, C., & Li, L. (2020). miR-149 regulates the proliferation and apoptosis of human colonic carcinoma cells by targeting FZD5. International Journal of Clinical and Experimental Pathology, 13(5), 889.

Thiele, S., Zimmer, A., Göbel, A., Rachner, T. D., Rother, S., Fuessel, S., ... & Hofbauer, L. C. (2018). Role of WNT5A receptors FZD5 and RYK in prostate cancer cells. Oncotarget, 9(43), 27293.

Dai, Y., Lu, H., Wang, S., Chang, S., Li, C., Huang, Z., ... & Ge, J. (2018). MicroRNA-216b actively modulates diabetic angiopathy through inverse regulation on FZD5. Gene, 658, 129-135.

Lin, J. F., Zeng, H., & Zhao, J. Q. (2018). MiR-212-5p regulates the proliferation and apoptosis of AML cells through targeting FZD5. Eur Rev Med Pharmacol Sci, 22(23), 8415-8422.

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For research use only. Not intended for any clinical use.

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