Mouse Anti-GNA12 Recombinant Antibody (5E9H9) (CBMAB-G4051-LY)

Name: Mouse Anti-GNA12 Recombinant Antibody (5E9H9)
SKU: CBMAB-G4051-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5E9H9

Application

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

guanine nucleotide binding protein, alpha 12

Entrez Gene ID

14673

UniProt ID

P27600

Alternative Names

AI414047; AI504261; Galpha12

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:22609986, PubMed:15525651, PubMed:15240885, PubMed:17565996, PubMed:12515866, PubMed:16787920, PubMed:16705036, PubMed:23762476, PubMed:27084452).

Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866, PubMed:16202387).

GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity).

GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996).

Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production (PubMed:23762476, PubMed:16787920, PubMed:16705036, PubMed:27084452).

Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920).

Together with NAPA promotes CDH5 localization to plasma membrane (PubMed:15980433).

May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).

Biological Process

Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Blood coagulation Source: ProtInc
G protein-coupled receptor signaling pathway Source: ProtInc
Negative regulation of vascular associated smooth muscle cell migration Source: Ensembl
Negative regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Regulation of fibroblast migration Source: UniProtKB
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB
Response to drug Source: Ensembl
Rho protein signal transduction Source: GO_Central

Cellular Location

Cell membrane; Lateral cell membrane; Cytoplasm. CDH1 enhances cell membrane localization.

Ha, J. H., Jayaraman, M., Yan, M., Dhanasekaran, P., Isidoro, C., Song, Y. S., & Dhanasekaran, D. N. (2021). Identification of GNA12‑driven gene signatures and key signaling networks in ovarian cancer. Oncology Letters, 22(4), 1-14.

Gismondi, A., Nanni, V., Monteleone, V., Colao, C., Di Marco, G., & Canini, A. (2021). Plant miR171 modulates mTOR pathway in HEK293 cells by targeting GNA12. Molecular Biology Reports, 48, 435-449.

Yun, J., Heo, M. J., Kim, Y. S., Kim, M. J., Moon, A., & Kim, S. G. (2020). Estrogen receptor alpha inhibits mesenchymal and amoeboidal movement of liver cancer cell via G protein subunit alpha 12. Authorea Preprints.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-GNA12 Recombinant Antibody (CBFYH-0402) (CAT#: CBMAB-H1272-FY)

Mouse Anti-GNA12 Recombinant Antibody (CBLG1-3195) (CAT#: CBMAB-G4052-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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