Mouse Anti-GOLPH3 Recombinant Antibody (CBFYH-0421) (CBMAB-H1292-FY)

Name: Mouse Anti-GOLPH3 Recombinant Antibody (CBFYH-0421)
SKU: CBMAB-H1292-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0421

Application

IHC-P, WB

Immunogen

Purified His-tagged GOLPH3 protein.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Golgi Phosphoprotein 3

Introduction

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Entrez Gene ID

64083

UniProt ID

Q9H4A6

Alternative Names

Golgi Phosphoprotein 3; Golgi Peripheral Membrane Protein 1, 34 KDa; Mitochondrial DNA Absence Factor; Golgi-Associated Protein; Coat Protein GPP34; Golgi Protein; Coat-Protein

Function

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

Biological Process

Asymmetric Golgi ribbon formation Source: CACAO
Cell adhesion molecule production Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to rapamycin Source: UniProtKB
Gene expression Source: UniProtKB
Glycoprotein biosynthetic process Source: UniProtKB
Golgi organization Source: UniProtKB
Golgi ribbon formation Source: CACAO
Golgi to plasma membrane protein transport Source: UniProtKB
Golgi vesicle budding Source: UniProtKB
Lamellipodium assembly Source: UniProtKB
Leukocyte tethering or rolling Source: UniProtKB
Negative regulation of apoptotic process Source: CACAO
Positive regulation of protein secretion Source: UniProtKB
Positive regulation of TOR signaling Source: UniProtKB
Protein retention in Golgi apparatus Source: UniProtKB
Protein secretion Source: UniProtKB
Regulation of mitochondrion organization Source: UniProtKB
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central

Cellular Location

Golgi stack membrane; Trans-Golgi network membrane; Endosome; Mitochondrion intermembrane space; Cell membrane. Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.

PTM

Phosphorylated.

Lowe, M. (2021). GOLPH3 keeps the Golgi residents at home. The Journal of cell biology, 220(10).

Welch, L. G., Peak-Chew, S. Y., Begum, F., Stevens, T. J., & Munro, S. (2021). GOLPH3 and GOLPH3L are broad-spectrum COPI adaptors for sorting into intra-Golgi transport vesicles. Journal of Cell Biology, 220(10).

Song, J. W., Zhu, J., Wu, X. X., Tu, T., Huang, J. Q., Chen, G. Z., ... & Gong, L. Y. (2021). GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer. Cell Death & Disease, 12(11), 976.

Sechi, S., Karimpour-Ghahnavieh, A., Frappaolo, A., Di Francesco, L., Piergentili, R., Schininà, E., ... & Giansanti, M. G. (2021). Identification of GOLPH3 partners in Drosophila unveils potential novel roles in tumorigenesis and neural disorders. Cells, 10(9), 2336.

Sechi, S., Frappaolo, A., Karimpour-Ghahnavieh, A., Piergentili, R., & Giansanti, M. G. (2020). Oncogenic Roles of GOLPH3 in the Physiopathology of Cancer. International Journal of Molecular Sciences, 21(3), 933.

Yu, T., An, Q., Cao, X. L., Yang, H., Cui, J., Li, Z. J., & Xiao, G. (2020). GOLPH3 inhibition reverses oxaliplatin resistance of colon cancer cells via suppression of PI3K/AKT/mTOR pathway. Life sciences, 260, 118294.

Lu, L. Q., Tang, M. Z., Qi, Z. H., Huang, S. F., He, Y. Q., Li, D. K., ... & Chen, L. X. (2020). Regulation of the Golgi apparatus via GOLPH3-mediated new selective autophagy. Life sciences, 253, 117700.

Kuna, R. S., & Field, S. J. (2019). GOLPH3: a Golgi phosphatidylinositol (4) phosphate effector that directs vesicle trafficking and drives cancer. Journal of lipid research, 60(2), 269-275.

Rahajeng, J., Kuna, R. S., Makowski, S. L., Tran, T. T., Buschman, M. D., Li, S., ... & Field, S. J. (2019). Efficient Golgi forward trafficking requires GOLPH3-driven, PI4P-dependent membrane curvature. Developmental cell, 50(5), 573-585.

Wu, S., Fu, J., Dong, Y., Yi, Q., Lu, D., Wang, W., ... & Zhou, X. (2018). GOLPH3 promotes glioma progression via facilitating JAK2–STAT3 pathway activation. Journal of neuro-oncology, 139, 269-279.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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