Rabbit Anti-GPBAR1 Recombinant Antibody (EG1351) (CBMAB-EN1629-LY)

Name: Rabbit Anti-GPBAR1 Recombinant Antibody (EG1351)
SKU: CBMAB-EN1629-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1351

Application

IF: 1:100~1:500 ELISA: 1:10000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPBAR.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled bile acid receptor 1

Introduction

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.

Entrez Gene ID

151306

UniProt ID

Q8TDU6

Alternative Names

BG37; TGR5; M-BAR; GPCR19; GPR131

Function

Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.

Biological Process

Cell surface bile acid receptor signaling pathway Source: UniProtKB
Cellular response to bile acid Source: UniProtKB
Positive regulation of cholangiocyte proliferation Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Regulation of bicellular tight junction assembly Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-19
Helical: 20-40
Cytoplasmic: 41-50
Helical: 51-71
Extracellular: 72-85
Helical: 86-106
Cytoplasmic: 107-125
Helical: 126-146
Extracellular: 147-165
Helical: 166-186
Cytoplasmic: 187-228
Helical: 229-249
Extracellular: 250-261
Helical: 262-282
Cytoplasmic: 283-330

Chen, T., Liu, H., Liu, Z., Li, K., Qin, R., Wang, Y., ... & Xu, Y. (2021). FGF19 and FGFR4 promotes the progression of gallbladder carcinoma in an autocrine pathway dependent on GPBAR1-cAMP-EGR1 axis. Oncogene, 40(30), 4941-4953.

Portincasa, P., Di Ciaula, A., Garruti, G., Vacca, M., De Angelis, M., & Wang, D. Q. H. (2020). Bile acids and GPBAR-1: Dynamic interaction involving genes, environment and gut microbiome. Nutrients, 12(12), 3709.

Lund, M. L., Sorrentino, G., Egerod, K. L., Kroone, C., Mortensen, B., Knop, F. K., ... & Petersen, N. (2020). L-cell differentiation is induced by bile acids through GPBAR1 and paracrine GLP-1 and serotonin signaling. Diabetes, 69(4), 614-623.

Keitel, V., Stindt, J., & Häussinger, D. (2019). Bile acid-activated receptors: GPBAR1 (TGR5) and other G protein-coupled receptors. Bile Acids and Their Receptors, 19-49.

Carino, A., Marchiano, S., Biagioli, M., Bucci, M., Vellecco, V., Brancaleone, V., ... & Fiorucci, S. (2019). Agonism for the bile acid receptor GPBAR1 reverses liver and vascular damage in a mouse model of steatohepatitis. The FASEB Journal, 33(2), 2809-2822.

Klindt, C., Reich, M., Hellwig, B., Stindt, J., Rahnenführer, J., Hengstler, J. G., ... & Keitel, V. (2019). The G protein-coupled bile acid receptor TGR5 (Gpbar1) modulates endothelin-1 signaling in liver. Cells, 8(11), 1467.

De Marino, S., Festa, C., Sepe, V., & Zampella, A. (2019). Chemistry and pharmacology of GPBAR1 and FXR selective agonists, dual agonists, and antagonists. Bile Acids and Their Receptors, 137-165.

Biagioli, M., Carino, A., Fiorucci, C., Marchianò, S., Di Giorgio, C., Roselli, R., ... & Fiorucci, S. (2019). GPBAR1 functions as gatekeeper for liver NKT cells and provides counterregulatory signals in mouse models of immune-mediated hepatitis. Cellular and Molecular Gastroenterology and Hepatology, 8(3), 447-473.

Keitel, V., & Häussinger, D. (2018, November). Role of TGR5 (GPBAR1) in liver disease. In Seminars in liver disease (Vol. 38, No. 04, pp. 333-339). Thieme Medical Publishers.

Chen, T., Reich, N. W., Bell, N., Finn, P. D., Rodriguez, D., Kohler, J., ... & Lewis, J. G. (2018). Design of gut-restricted thiazolidine agonists of G protein-coupled bile acid receptor 1 (GPBAR1, TGR5). Journal of Medicinal Chemistry, 61(17), 7589-7613.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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