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Rabbit Anti-GPBAR1 Recombinant Antibody (EG1351) (CBMAB-EN1629-LY)

The product is antibody recognizes GPBAR. The antibody EG1351 immunoassay techniques such as: IF: 1:100~1:500 ELISA: 1:10000.
See all GPBAR1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG1351
Antibody Isotype
IgG
Application
IF: 1:100~1:500 ELISA: 1:10000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPBAR.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled bile acid receptor 1
Introduction
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Entrez Gene ID
151306
UniProt ID
Q8TDU6
Alternative Names
BG37; TGR5; M-BAR; GPCR19; GPR131
Function
Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
Biological Process
Cell surface bile acid receptor signaling pathway Source: UniProtKB
Cellular response to bile acid Source: UniProtKB
Positive regulation of cholangiocyte proliferation Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Regulation of bicellular tight junction assembly Source: Ensembl
Cellular Location
Cell membrane
Topology
Extracellular: 1-19
Helical: 20-40
Cytoplasmic: 41-50
Helical: 51-71
Extracellular: 72-85
Helical: 86-106
Cytoplasmic: 107-125
Helical: 126-146
Extracellular: 147-165
Helical: 166-186
Cytoplasmic: 187-228
Helical: 229-249
Extracellular: 250-261
Helical: 262-282
Cytoplasmic: 283-330

Chen, T., Liu, H., Liu, Z., Li, K., Qin, R., Wang, Y., ... & Xu, Y. (2021). FGF19 and FGFR4 promotes the progression of gallbladder carcinoma in an autocrine pathway dependent on GPBAR1-cAMP-EGR1 axis. Oncogene, 40(30), 4941-4953.

Portincasa, P., Di Ciaula, A., Garruti, G., Vacca, M., De Angelis, M., & Wang, D. Q. H. (2020). Bile acids and GPBAR-1: Dynamic interaction involving genes, environment and gut microbiome. Nutrients, 12(12), 3709.

Lund, M. L., Sorrentino, G., Egerod, K. L., Kroone, C., Mortensen, B., Knop, F. K., ... & Petersen, N. (2020). L-cell differentiation is induced by bile acids through GPBAR1 and paracrine GLP-1 and serotonin signaling. Diabetes, 69(4), 614-623.

Keitel, V., Stindt, J., & Häussinger, D. (2019). Bile acid-activated receptors: GPBAR1 (TGR5) and other G protein-coupled receptors. Bile Acids and Their Receptors, 19-49.

Carino, A., Marchiano, S., Biagioli, M., Bucci, M., Vellecco, V., Brancaleone, V., ... & Fiorucci, S. (2019). Agonism for the bile acid receptor GPBAR1 reverses liver and vascular damage in a mouse model of steatohepatitis. The FASEB Journal, 33(2), 2809-2822.

Klindt, C., Reich, M., Hellwig, B., Stindt, J., Rahnenführer, J., Hengstler, J. G., ... & Keitel, V. (2019). The G protein-coupled bile acid receptor TGR5 (Gpbar1) modulates endothelin-1 signaling in liver. Cells, 8(11), 1467.

De Marino, S., Festa, C., Sepe, V., & Zampella, A. (2019). Chemistry and pharmacology of GPBAR1 and FXR selective agonists, dual agonists, and antagonists. Bile Acids and Their Receptors, 137-165.

Biagioli, M., Carino, A., Fiorucci, C., Marchianò, S., Di Giorgio, C., Roselli, R., ... & Fiorucci, S. (2019). GPBAR1 functions as gatekeeper for liver NKT cells and provides counterregulatory signals in mouse models of immune-mediated hepatitis. Cellular and Molecular Gastroenterology and Hepatology, 8(3), 447-473.

Keitel, V., & Häussinger, D. (2018, November). Role of TGR5 (GPBAR1) in liver disease. In Seminars in liver disease (Vol. 38, No. 04, pp. 333-339). Thieme Medical Publishers.

Chen, T., Reich, N. W., Bell, N., Finn, P. D., Rodriguez, D., Kohler, J., ... & Lewis, J. G. (2018). Design of gut-restricted thiazolidine agonists of G protein-coupled bile acid receptor 1 (GPBAR1, TGR5). Journal of Medicinal Chemistry, 61(17), 7589-7613.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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