Rabbit Anti-GPR132 Recombinant Antibody (EG1372) (CBMAB-EN1651-LY)

Name: Rabbit Anti-GPR132 Recombinant Antibody (EG1372)
SKU: CBMAB-EN1651-LY
Rating: 5 (1 reviews)

See all GPR132 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1372

Application

WB: 1:500~1:1000 ELISA: 1:5000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR132.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G Protein-Coupled Receptor 132

Introduction

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Entrez Gene ID

29933

UniProt ID

Q9UNW8

Alternative Names

G Protein-Coupled Receptor 132; G2 Accumulation Protein; G2A; Probable G-Protein Coupled Receptor 132; G2 Accumulation;

Function

May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity.

Biological Process

G1/S transition of mitotic cell cycle Source: GO_Central
Negative regulation of G2/M transition of mitotic cell cycle Source: GO_Central

Cellular Location

Cell membrane. Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface (By similarity).

Topology

Extracellular: 1-45
Helical: 46-68
Cytoplasmic: 69-79
Helical: 80-102
Extracellular: 103-116
Helical: 117-138
Cytoplasmic: 139-158
Helical: 159-178
Extracellular: 179-197
Helical: 198-220
Cytoplasmic: 221-246
Helical: 247-269
Extracellular: 270-288
Helical: 289-311
Cytoplasmic: 312-380

Imenez Silva, P. H., Camara, N. O., & Wagner, C. A. (2022). Role of proton-activated G protein-coupled receptors in pathophysiology. American Journal of Physiology-Cell Physiology, 323(2), C400-C414.

Shen, S., Guo, Q., & Zhan, S. (2021). MicroRNA-612 regulates the proliferation and epithelial-to-mesenchymal transition of human colon cancer cells via G protein-coupled receptor 132 (GPR132). Tropical Journal of Pharmaceutical Research, 20(7), 1345-1350.

Lahvic, J. L., Ammerman, M., Li, P., Blair, M. C., Stillman, E. R., Fast, E. M., ... & Zon, L. I. (2018). Specific oxylipins enhance vertebrate hematopoiesis via the receptor GPR132. Proceedings of the National Academy of Sciences, 115(37), 9252-9257.

Vangaveti, V., Shashidhar, V., Collier, F., Hodge, J., Rush, C., Malabu, U., ... & Kennedy, R. L. (2018). 9-and 13-HODE regulate fatty acid binding protein-4 in human macrophages, but does not involve HODE/GPR132 axis in PPAR-γ regulation of FABP4. Therapeutic advances in endocrinology and metabolism, 9(5), 137-150.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-GPR132 Recombinant Antibody (EG1372)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry