Rabbit Anti-GPR135 Recombinant Antibody (EG1374) (CBMAB-EN1653-LY)

The product is antibody recognizes GPR135. The antibody EG1374 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:1000.
See all GPR135 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

EG1374

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:1000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR135.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G protein-coupled receptor 135

Entrez Gene ID

Human	64582
Mouse	238252
Rat	314213

UniProt ID

Human	Q8IZ08
Mouse	Q7TQP2
Rat	Q7TQN7

Alternative Names

HUMNPIIY20

Function

Orphan receptor. Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538).

Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).

Biological Process

G protein-coupled receptor signaling pathway Source: GO_Central

Cellular Location

Endosome membrane; Cell membrane. Colocalizes with ARRB2/beta-arrestin-2 in the endosome.

Topology

Extracellular: 1-12
Helical: 13-33
Cytoplasmic: 34-37
Helical: 38-58
Extracellular: 59-81
Helical: 82-102
Cytoplasmic: 103-125
Helical: 126-146
Extracellular: 147-164
Helical: 165-185
Cytoplasmic: 186-226
Helical: 227-247
Extracellular: 248-262
Helical: 263-283
Cytoplasmic: 284-494

References

Gutiérrez-Ruiz, J. R., Villafaña, S., Ruiz-Hernández, A., Viruette-Pontigo, D., Menchaca-Cervantes, C., Aguayo-Cerón, K. A., ... & Romero-Nava, R. (2022). Expression profiles of GPR21, GPR39, GPR135, and GPR153 orphan receptors in different cancers. Nucleosides, Nucleotides & Nucleic Acids, 41(2), 123-136.

Watkins, L. R., & Orlandi, C. (2020). Orphan G protein coupled receptors in affective disorders. Genes, 11(6), 694.

Oishi, A., Cecon, E., & Jockers, R. (2018). Melatonin receptor signaling: impact of receptor oligomerization on receptor function. International Review of Cell and Molecular Biology, 338, 59-77.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-GPR135 Recombinant Antibody (1H5)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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