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  • Rabbit Anti-GPR135 Recombinant Antibody (EG1374)

Rabbit Anti-GPR135 Recombinant Antibody (EG1374) (CBMAB-EN1653-LY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
EG1374
Application
WB: 1:500~1:1000 ELISA: 1:1000
Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPR135.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
G protein-coupled receptor 135
Entrez Gene ID
Human64582
Mouse238252
Rat314213
UniProt ID
HumanQ8IZ08
MouseQ7TQP2
RatQ7TQN7
Alternative Names
HUMNPIIY20
Function
Orphan receptor.

Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).
Biological Process
G protein-coupled receptor signaling pathway Source: GO_Central
Topology
Extracellular: 1-12
Helical: 13-33
Cytoplasmic: 34-37
Helical: 38-58
Extracellular: 59-81
Helical: 82-102
Cytoplasmic: 103-125
Helical: 126-146
Extracellular: 147-164
Helical: 165-185
Cytoplasmic: 186-226
Helical: 227-247
Extracellular: 248-262
Helical: 263-283
Cytoplasmic: 284-494

Gutiérrez-Ruiz, J. R., Villafaña, S., Ruiz-Hernández, A., Viruette-Pontigo, D., Menchaca-Cervantes, C., Aguayo-Cerón, K. A., ... & Romero-Nava, R. (2022). Expression profiles of GPR21, GPR39, GPR135, and GPR153 orphan receptors in different cancers. Nucleosides, Nucleotides & Nucleic Acids, 41(2), 123-136.

Watkins, L. R., & Orlandi, C. (2020). Orphan G protein coupled receptors in affective disorders. Genes, 11(6), 694.

Oishi, A., Cecon, E., & Jockers, R. (2018). Melatonin receptor signaling: impact of receptor oligomerization on receptor function. International Review of Cell and Molecular Biology, 338, 59-77.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-GPR135 Recombinant Antibody (1H5) (CAT#: CBMAB-G4631-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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