Rabbit Anti-GPR149 Recombinant Antibody (EG1381) (CBMAB-EN1660-LY)

Name: Rabbit Anti-GPR149 Recombinant Antibody (EG1381)
SKU: CBMAB-EN1660-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1381

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR149.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G Protein-Coupled Receptor 149

Introduction

This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]

Entrez Gene ID

344758

UniProt ID

Q86SP6

Alternative Names

G Protein-Coupled Receptor 149; PGR10; Probable G-Protein Coupled Receptor 149; G Protein-Coupled Receptor PGR10; G-Protein Coupled Receptor PGR10; IEDA; R35;

Function

Orphan receptor.

Biological Process

Antral ovarian follicle growth Source: Ensembl
Negative regulation of ovulation Source: Ensembl
Neuropeptide signaling pathway Source: GO_Central
Preantral ovarian follicle growth Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-35
Helical: 36-56
Cytoplasmic: 57-69
Helical: 70-90
Extracellular: 91-109
Helical: 110-132
Cytoplasmic: 133-155
Helical: 156-176
Extracellular: 177-189
Helical: 190-210
Cytoplasmic: 211-310
Helical: 311-331
Extracellular: 332-342
Helical: 343-363
Cytoplasmic: 364-731

Ruiz-Hernández, A., Cabrera-Becerra, S., Vera-Juárez, G., Hong, E., Fengyang, H., Arauz, J., & Villafaña, S. (2020). Diabetic nephropathy produces alterations in the tissue expression profile of the orphan receptors GPR149, GPR153, GPR176, TAAR3, TAAR5 and TAAR9 in Wistar rats. Nucleosides, nucleotides & nucleic acids, 39(8), 1150-1161.

Kim, Y. J., Jang, W., Piao, X. M., Yoon, H. Y., Byun, Y. J., Kim, J. S., ... & Kim, W. J. (2019). ZNF492 and GPR149 methylation patterns as prognostic markers for clear cell renal cell carcinoma: Array‑based DNA methylation profiling. Oncology Reports, 42(1), 453-460.

Egerod, K. L., Petersen, N., Timshel, P. N., Rekling, J. C., Wang, Y., Liu, Q., ... & Gautron, L. (2018). Profiling of G protein-coupled receptors in vagal afferents reveals novel gut-to-brain sensing mechanisms. Molecular metabolism, 12, 62-75.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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