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Rabbit Anti-GPR22 Recombinant Antibody (EG1397) (CBMAB-EN1676-LY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
EG1397
Application
IF: 1:100~1:500 ELISA: 1:1000
Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPR22.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
G protein-coupled receptor 22
Introduction
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein.
Entrez Gene ID
Human2845
Mouse73010
UniProt ID
HumanQ99680
MouseQ8BZL4
Function
Orphan G-protein coupled receptor. Seems to act through a G(i)/G(o) mediated pathway (PubMed:18539757).

May be involved in ciliogenesis (By similarity).
Biological Process
Cell projection organization Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 1-45
Helical: 46-66
Cytoplasmic: 67-85
Helical: 86-106
Extracellular: 107-115
Helical: 116-136
Cytoplasmic: 137-156
Helical: 157-177
Extracellular: 178-208
Helical: 209-229
Cytoplasmic: 230-315
Helical: 316-336
Extracellular: 337-349
Helical: 350-370
Cytoplasmic: 371-433

Watkins, L. R., & Orlandi, C. (2021). In vitro profiling of orphan G protein coupled receptor (GPCR) constitutive activity. British Journal of Pharmacology, 178(15), 2963-2975.

Atanes, P., Ashik, T., & Persaud, S. J. (2021). Obesity-induced changes in human islet G protein-coupled receptor expression: Implications for metabolic regulation. Pharmacology & Therapeutics, 228, 107928.

Guns, L. A., Lories, R., & Cailotto, F. (2018). G-proteïne-gekoppelde-receptor 22 (GPR22): een nieuw doelwit in osteoartrose?.

Egerod, K. L., Petersen, N., Timshel, P. N., Rekling, J. C., Wang, Y., Liu, Q., ... & Gautron, L. (2018). Profiling of G protein-coupled receptors in vagal afferents reveals novel gut-to-brain sensing mechanisms. Molecular metabolism, 12, 62-75.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-GPR22 Recombinant Antibody (CBFYH-0455) (CAT#: CBMAB-H0498-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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