Rabbit Anti-GPR52 Recombinant Antibody (EG1403) (CBMAB-EN1682-LY)

The product is antibody recognizes GPR52. The antibody EG1403 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all GPR52 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

EG1403

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from C-terminal of human GPR52.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G Protein-Coupled Receptor 52

Introduction

Members of the G protein-coupled receptor (GPR) family play important roles in signal transduction from the external environment to the inside of the cell.[supplied by OMIM, Jul 2002]

Entrez Gene ID

9293

UniProt ID

Q9Y2T5

Alternative Names

G Protein-Coupled Receptor 52;

Function

Gs-coupled receptor activated by antipsychotics reserpine leading to an increase in intracellular cAMP and its internalization (PubMed:24587241).

May play a role in locomotor activity through modulation of dopamine, NMDA and ADORA2A-induced locomotor activity. These behavioral changes are accompanied by modulation of the dopamine receptor signaling pathway in striatum (PubMed:24587241).

Modulates HTT level via cAMP-dependent but PKA independent mechanisms throught activation of RAB39B that translocates HTT to the endoplasmic reticulum, thus avoiding proteasome degradation (PubMed:25738228).

Biological Process

Cellular response to light stimulus Source: GO_Central
G protein-coupled receptor signaling pathway Source: GO_Central
Locomotory behavior Source: UniProtKB
Phototransduction Source: GO_Central
Response to drug Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 1-44
Helical: 45-65
Cytoplasmic: 66-87
Helical: 88-108
Extracellular: 109-116
Helical: 117-137
Cytoplasmic: 138-159
Helical: 160-180
Extracellular: 181-203
Helical: 204-224
Cytoplasmic: 225-265
Helical: 266-286
Extracellular: 287-296
Helical: 297-317
Cytoplasmic: 318-361

References

Stott, L. A., Brighton, C. A., Brown, J., Mould, R., Bennett, K. A., Newman, R., ... & Watson, S. P. (2021). Characterisation of inverse agonism of the orphan-G protein-coupled receptor GPR52 by cannabinoid ligands Cannabidiol and O-1918. Heliyon, 7(6), e07201.

Russell, B., Barrus, M. M., Tremblay, M., Ma, L., Hrelja, K., Wong, C., ... & Winstanley, C. A. (2021). GPR52 agonists attenuate ropinirole-induced preference for uncertain outcomes. Behavioral Neuroscience, 135(1), 8.

Lin, X., Li, M., Wang, N., Wu, Y., Luo, Z., Guo, S., ... & Xu, F. (2020). Structural basis of ligand recognition and self-activation of orphan GPR52. Nature, 579(7797), 152-157.

Wang, P., Felsing, D. E., Chen, H., Stutz, S. J., Murphy, R. E., Cunningham, K. A., ... & Zhou, J. (2020). Discovery of potent and brain-penetrant GPR52 agonist that suppresses psychostimulant behavior. Journal of medicinal chemistry, 63(22), 13951-13972.

Alavi, M. S., Shamsizadeh, A., Azhdari-Zarmehri, H., & Roohbakhsh, A. (2018). Orphan G protein-coupled receptors: The role in CNS disorders. Biomedicine & Pharmacotherapy, 98, 222-232.

Nakahata, T., Tokumaru, K., Ito, Y., Ishii, N., Setoh, M., Shimizu, Y., ... & Aso, K. (2018). Design and synthesis of 1-(1-benzothiophen-7-yl)-1H-pyrazole, a novel series of G protein-coupled receptor 52 (GPR52) agonists. Bioorganic & Medicinal Chemistry, 26(8), 1598-1608.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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