Rabbit Anti-GPRC6A Recombinant Antibody (EG1416) (CBMAB-EN1695-LY)

Name: Rabbit Anti-GPRC6A Recombinant Antibody (EG1416)
SKU: CBMAB-EN1695-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1416

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPC6A.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G Protein-Coupled Receptor Class C Group 6 Member A

Introduction

Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]

Entrez Gene ID

222545

UniProt ID

Q5T6X5

Alternative Names

G Protein-Coupled Receptor Class C Group 6 Member A; G Protein-Coupled Receptor, Family C, Group 6, Member A; G-Protein Coupled Receptor GPCR33; G Protein-Coupled Receptor, Class C, Group 6, Member A; G-Protein Coupled Receptor Family C Group 6 Member A; Seven Transmembrane Helix Receptor;

Function

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids.

Biological Process

Response to amino acid Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 19-594
Helical: 595-615
Cytoplasmic: 616-631
Helical: 632-652
Extracellular: 653-669
Helical: 670-690
Cytoplasmic: 691-704
Helical: 705-725
Extracellular: 726-748
Helical: 749-769
Cytoplasmic: 770-782
Helical: 783-803
Extracellular: 804-810
Helical: 811-831
Cytoplasmic: 832-926

PTM

N-glycosylated.

Hou, Q., Huang, J., Xiong, X., Guo, Y., & Zhang, B. (2022). Role of nutrient-sensing receptor GPRC6A in regulating colonic group 3 innate lymphoid cells and inflamed mucosal healing. Journal of Crohn's and Colitis, 16(8), 1293-1305.

Zhang, M., Nie, X., Yuan, Y., Wang, Y., Ma, X., Yin, J., & Bao, Y. (2021). Osteocalcin alleviates nonalcoholic fatty liver disease in mice through GPRC6A. International Journal of Endocrinology, 2021, 1-10.

Pi, M., Nishimoto, S. K., & Darryl Quarles, L. (2021). Explaining divergent observations regarding osteocalcin/GPRC6A endocrine signaling. Endocrinology, 162(4), bqab011.

Pi, M., Xu, F., Ye, R., Nishimoto, S. K., Kesterson, R. A., Williams, R. W., ... & Quarles, L. D. (2020). Humanized GPRC6A KGKY is a gain-of-function polymorphism in mice. Scientific Reports, 10(1), 11143.

Jørgensen, C. V., & Bräuner‐Osborne, H. (2020). Pharmacology and physiological function of the orphan GPRC6A receptor. Basic & Clinical Pharmacology & Toxicology, 126, 77-87.

Pi, M., Xu, F., Ye, R., Nishimoto, S. K., Williams, R. W., Lu, L., & Darryl Quarles, L. (2020). Role of GPRC6A in regulating hepatic energy metabolism in mice. Scientific Reports, 10(1), 1-12.

Diaz‑Franco, M. C., Franco‑Diaz de Leon, R., & Villafan‑Bernal, J. R. (2019). Osteocalcin‑GPRC6A: An update of its clinical and biological multi‑organic interactions. Molecular Medicine Reports, 19(1), 15-22.

Jørgensen, C. V., Gasparini, S. J., Tu, J., Zhou, H., Seibel, M. J., & Bräuner-Osborne, H. (2019). Metabolic and skeletal homeostasis are maintained in full locus GPRC6A knockout mice. Scientific reports, 9(1), 5995.

Kalyvianaki, K., Panagiotopoulos, A. A., Malamos, P., Moustou, E., Tzardi, M., Stathopoulos, E. N., ... & Kampa, M. (2019). Membrane androgen receptors (OXER1, GPRC6A AND ZIP9) in prostate and breast cancer: A comparative study of their expression. Steroids, 142, 100-108.

Pi, M., Kapoor, K., Ye, R., Hwang, D. J., Miller, D. D., Smith, J. C., ... & Quarles, L. D. (2018). Computationally identified novel agonists for GPRC6A. PloS one, 13(4), e0195980.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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