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Mouse Anti-GRB7 Recombinant Antibody (1C7) (CBMAB-0595-CN)

This product is a mouse antibody that recognizes GRB7 of human. The antibody 1C7 can be used for immunoassay techniques such as: ELISA, MA.
See all GRB7 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C7
Antibody Isotype
IgG
Application
ELISA, MA

Basic Information

Immunogen
Recombinant protein SH2 domain of GRB7 (aa. 417-532)
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Growth Factor Receptor Bound Protein 7
Introduction
GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular direct link to the Ras proto-oncogene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3, as well as activation of HRAS. This protein plays a role in signal transduction in response to EGF and in the regulation of cell proliferation and cell migration.
Entrez Gene ID
2886
UniProt ID
Q14451
Alternative Names
Growth factor receptor bound protein 7
Function
Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).
Biological Process
Epidermal growth factor receptor signaling pathway Source: ProtInc
Negative regulation of translation Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Stress granule assembly Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; Focal adhesion; Cytoplasmic granule; Cell projection. Predominantly cytoplasmic. Detected in stress granules, where mRNA is stored under stress conditions.
PTM
Phosphorylated on serine and threonine residues in response to heregulin. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues in response to NTN1 signaling. Phosphorylation promotes stress granule disassembly during recovery after cellular stress (By similarity). Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases. Tyrosine phosphorylation is essential for activation of down-stream protein kinases.

Chauhan, A., & Kalra, S. (2023). Erb-b2 receptor tyrosine kinase 2 interaction with growth factor receptor bound protein 7 acts as a molecular switch to activate non-small cell lung cancer: An in silico prediction. Biomedical and Biotechnology Research Journal (BBRJ), 7(1), 111.

Xu, Q., Liu, Z., Zhu, Z. Q., Fan, Y., Chen, R., Xie, X. H., & Cheng, M. (2021). Knockdown of growth factor receptor bound protein 7 suppresses angiogenesis by inhibiting the secretion of vascular endothelial growth factor A in ovarian cancer cells. Bioengineered, 12(2), 12179-12190.

Zheng, Y., Pei, Y., Yang, L., Zeng, Z., Wang, J., Xie, G., ... & Yuan, J. (2020). Upregulated GRB7 promotes proliferation and tumorigenesis of Bladder Cancer via Phospho-AKT Pathway. International Journal of Biological Sciences, 16(16), 3221.

Tang, H., Yang, P., Yang, X., Peng, S., Hu, X. E., & Bao, G. (2020). Growth factor receptor bound protein-7 regulates proliferation, cell cycle, and mitochondrial apoptosis of thyroid cancer cells via MAPK/ERK signaling. Molecular and Cellular Biochemistry, 472, 209-218.

Gotovac, J. R., Liu, D. S., Yates, M. J., Milne, J. V., Macpherson, A. A., Simpson, K. J., ... & Clemons, N. J. (2020). GRB7 is an oncogenic driver and potential therapeutic target in oesophageal adenocarcinoma. The Journal of Pathology, 252(3), 317-329.

Chu, P. Y., Tai, Y. L., & Shen, T. L. (2019). Grb7, a critical mediator of EGFR/ErbB signaling, in cancer development and as a potential therapeutic target. Cells, 8(5), 435.

Tang, Y., Yang, S., Wang, M., Liu, D., Liu, Y., Zhang, Y., & Zhang, Q. (2019). Epigenetically altered miR‑193a‑3p promotes HER2 positive breast cancer aggressiveness by targeting GRB7. International Journal of Molecular Medicine, 43(6), 2352-2360.

Chen, K., Liu, M. X., Mak, C. S. L., Yung, M. M. H., Leung, T. H. Y., Xu, D., ... & Chan, D. W. (2018). Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways. Theranostics, 8(2), 423.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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