Mouse Anti-GRK1 Recombinant Antibody (CBFYH-0484) (CBMAB-H1354-FY)

Name: Mouse Anti-GRK1 Recombinant Antibody (CBFYH-0484)
SKU: CBMAB-H1354-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0484

Application

WB, IF

Immunogen

Full length human GRK1

Specificity

Human, Mouse, Rat, Cattle, Chicken

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor kinase 1

Introduction

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).

Entrez Gene ID

Human	6011
Mouse	24013
Rat	81760
Cattle	281457
Chicken	395431

UniProt ID

Human	Q15835
Mouse	Q9WVL4
Rat	Q63651
Cattle	P28327
Chicken	O73685

Alternative Names

G Protein-Coupled Receptor Kinase 1; Rhodopsin Kinase; EC 2.7.11.14; RHOK; RK; EC 2.7.11; GPRK1

Function

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:15946941).

This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity).

May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).

Biological Process

Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Rhodopsin mediated signaling pathway Source: ProtInc
Visual perception Source: ProtInc

Cellular Location

Membrane; Photoreceptor outer segment. Subcellular location is not affected by light or dark conditions.

Involvement in disease

Night blindness, congenital stationary, Oguchi type 2 (CSNBO2):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation.

PTM

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity.

Millette, M. A., Roy, S., & Salesse, C. (2022). Farnesylation and lipid unsaturation are critical for the membrane binding of the C-terminal segment of G-Protein Receptor Kinase 1. Colloids and Surfaces B: Biointerfaces, 211, 112315.

Gulati, S., & Palczewski, K. (2022). Structural view of G protein-coupled receptor signaling in the retinal rod outer segment. Trends in Biochemical Sciences.

Chen, Q., Plasencia, M., Li, Z., Mukherjee, S., Patra, D., Chen, C. L., ... & Tesmer, J. J. (2021). Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature, 595(7868), 600-605.

Sulon, S. M., & Benovic, J. L. (2021). Targeting G protein–coupled receptor kinases to G protein–coupled receptors. Current opinion in endocrine and metabolic research, 16, 56-65.

Benovic, J. L. (2021). Historical perspective of the G protein-coupled receptor kinase family. Cells, 10(3), 555.

Kolesnikov, A. V., Chrispell, J. D., Osawa, S., Kefalov, V. J., & Weiss, E. R. (2020). Phosphorylation at Serine 21 in G protein-coupled receptor kinase 1 (GRK1) is required for normal kinetics of dark adaption in rod but not cone photoreceptors. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 34(2), 2677.

Yu, S., Sun, L., Jiao, Y., & Lee, L. T. O. (2018). The role of G protein-coupled receptor kinases in cancer. International journal of biological sciences, 14(2), 189.

Komolov, K. E., & Benovic, J. L. (2018). G protein-coupled receptor kinases: Past, present and future. Cellular signalling, 41, 17-24.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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