Mouse Anti-GRK2 Recombinant Antibody (5D5) (CBMAB-G4989-LY)

Name: Mouse Anti-GRK2 Recombinant Antibody (5D5)
SKU: CBMAB-G4989-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5D5

Application

ICC/IF, IHC, WB

Immunogen

Purified recombinant human sARK1

Specificity

Human, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor kinase 2

Introduction

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Entrez Gene ID

Human	156
Rat	25238

UniProt ID

Human	P25098
Rat	P26817

Alternative Names

G Protein-Coupled Receptor Kinase 2; EC 2.7.11.15; Beta-ARK-1; ADRBK1; BARK1; Adrenergic; Beta; Receptor Kinase 1; G-Protein Coupled Receptor Kinase 2;

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed:19715378).

Key regulator of LPAR1 signaling (PubMed:19306925).

Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed:19306925).

Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925).

Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).

Inhibits relaxation of airway smooth muscle in response to blue light (PubMed:30284927).

Biological Process

Cardiac muscle contraction Source: BHF-UCL
Desensitization of G protein-coupled receptor signaling pathway Source: BHF-UCL
G protein-coupled acetylcholine receptor signaling pathway Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: GO_Central
Heart development Source: Ensembl
Negative regulation of relaxation of smooth muscle Source: UniProtKB
Negative regulation of striated muscle contraction Source: BHF-UCL
Negative regulation of the force of heart contraction by chemical signal Source: BHF-UCL
Peptidyl-serine phosphorylation Source: BHF-UCL
Peptidyl-threonine phosphorylation Source: Ensembl
Positive regulation of catecholamine secretion Source: BHF-UCL
Protein phosphorylation Source: GO_Central
Receptor internalization Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Regulation of the force of heart contraction Source: GO_Central
Tachykinin receptor signaling pathway Source: BHF-UCL
Viral entry into host cell Source: CACAO
Viral genome replication Source: CACAO

Cellular Location

Cell membrane; Cytoplasm

Davis, K., Mitchell, C., Weissenfels, O., Bai, J., Raizen, D. M., Ailion, M., & Topalidou, I. (2023). G protein-coupled receptor kinase-2 (GRK-2) controls exploration through neuropeptide signaling in Caenorhabditis elegans. PLoS genetics, 19(1), e1010613.

Crudden, C., Shibano, T., Song, D., Dragomir, M. P., Cismas, S., Serly, J., ... & Girnita, L. (2021). Inhibition of G Protein–Coupled Receptor Kinase 2 Promotes Unbiased Downregulation of IGF1 Receptor and Restrains Malignant Cell GrowthUnbiased IGF1R Downregulation through GRK2 Inhibition. Cancer research, 81(2), 501-514.

Xu, G., Gaul, M. D., Liu, Z., DesJarlais, R. L., Qi, J., Wang, W., ... & Spurlino, J. C. (2020). Hit-to-lead optimization and discovery of a potent, and orally bioavailable G protein coupled receptor kinase 2 (GRK2) inhibitor. Bioorganic & Medicinal Chemistry Letters, 30(23), 127602.

Kang, J. H., Toita, R., Kawano, T., Murata, M., & Asai, D. (2020). Design of substrates and inhibitors of G protein-coupled receptor kinase 2 (GRK2) based on its phosphorylation reaction. Amino Acids, 52, 863-870.

Gambardella, J., Sorriento, D., Bova, M., Rusciano, M., Loffredo, S., Wang, X., ... & Ciccarelli, M. (2020). Role of endothelial G protein-coupled receptor kinase 2 in angioedema. Hypertension, 76(5), 1625-1636.

Murga, C., Arcones, A. C., Cruces-Sande, M., Briones, A. M., Salaices, M., & Mayor Jr, F. (2019). G protein-coupled receptor kinase 2 (GRK2) as a potential therapeutic target in cardiovascular and metabolic diseases. Frontiers in pharmacology, 10, 112.

Penela, P., Ribas, C., Sánchez-Madrid, F., & Mayor, F. (2019). G protein-coupled receptor kinase 2 (GRK2) as a multifunctional signaling hub. Cellular and Molecular Life Sciences, 76, 4423-4446.

Mayor Jr, F., Cruces-Sande, M., Arcones, A. C., Vila-Bedmar, R., Briones, A. M., Salaices, M., & Murga, C. (2018). G protein-coupled receptor kinase 2 (GRK2) as an integrative signalling node in the regulation of cardiovascular function and metabolic homeostasis. Cellular Signalling, 41, 25-32.

Cruces-Sande, M., Vila-Bedmar, R., Arcones, A. C., Gonzalez-Rodriguez, A., Rada, P., Gutierrez-de-Juan, V., ... & Murga, C. (2018). Involvement of G protein-coupled receptor kinase 2 (GRK2) in the development of non-alcoholic steatosis and steatohepatitis in mice and humans. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(12), 3655-3667.

Pfleger, J., Gross, P., Johnson, J., Carter, R. L., Gao, E., Tilley, D. G., ... & Koch, W. J. (2018). G protein-coupled receptor kinase 2 contributes to impaired fatty acid metabolism in the failing heart. Journal of molecular and cellular cardiology, 123, 108-117.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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