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Rabbit Anti-GRK5 Recombinant Antibody (EG1446) (CBMAB-EN1727-LY)

The product is antibody recognizes GRK5. The antibody EG1446 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all GRK5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1446
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GRK5.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor kinase 5
Introduction
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs).
Entrez Gene ID
Human2869
Mouse14773
Rat59075
UniProt ID
HumanP34947
MouseQ8VEB1
RatQ62833
Alternative Names
GPRK5; FP2025
Function
Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Biological Process
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Apoptotic process Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of cell population proliferation Source: CACAO
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of cell cycle Source: CACAO
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of signal transduction Source: GO_Central
Tachykinin receptor signaling pathway Source: BHF-UCL
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Cell membrane; Nucleus; Cytoplasm. Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca2+/calmodulin-dependent fashion.
PTM
Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.

De Lucia, C., Grisanti, L. A., Borghetti, G., Piedepalumbo, M., Ibetti, J., Lucchese, A. M., ... & Koch, W. J. (2022). G protein-coupled receptor kinase 5 (GRK5) contributes to impaired cardiac function and immune cell recruitment in post-ischemic heart failure. Cardiovascular Research, 118(1), 169-183.

Downes, K., Zhao, X., Gleadall, N. S., McKinney, H., Kempster, C., Batista, J., ... & Ma, P. (2022). G protein–coupled receptor kinase 5 regulates thrombin signaling in platelets via PAR-1. Blood Advances, 6(7), 2319-2330.

Toya, M., Akasaki, Y., Sueishi, T., Kurakazu, I., Kuwahara, M., Uchida, T., ... & Nakashima, Y. (2021). G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis. Scientific reports, 11(1), 10481.

Guimarães, T. R., Swanson, E., Kofler, J., & Thathiah, A. (2021). G protein‐coupled receptor kinases are associated with Alzheimer's disease pathology. Neuropathology and applied neurobiology, 47(7), 942-957.

Sok, V., Jacinto, A. Z., Peng, N., Eldemerdash, M., Le, L., Tran, P. D., ... & So, C. H. (2021). G protein coupled receptor kinase 5 modifies the nucleolar stress response activated by actinomycin D. Biochemistry and Cell Biology, 99(4), 508-518.

Lagman, J., Sayegh, P., Lee, C. S., Sulon, S. M., Jacinto, A. Z., Sok, V., ... & So, C. H. (2019). G protein-coupled receptor kinase 5 modifies cancer cell resistance to paclitaxel. Molecular and Cellular Biochemistry, 461, 103-118.

Rowlands, R. A., Cato, M. C., Waldschmidt, H. V., Bouley, R. A., Chen, Q., Avramova, L., ... & White, A. D. (2019). Structure-based design of selective, covalent G protein-coupled receptor kinase 5 inhibitors. ACS Medicinal Chemistry Letters, 10(12), 1628-1634.

Yeh, S. T., Zambrano, C. M., Koch, W. J., & Purcell, N. H. (2018). PH domain leucine-rich repeat protein phosphatase 2 (PHLPP2) regulates G-protein–coupled receptor kinase 5 (GRK5)-induced cardiac hypertrophy in vitro. Journal of Biological Chemistry, 293(21), 8056-8064.

Lutz, S. Z., Falcenberg, M., Machicao, F., Peter, A., Kächele, M., Randrianarisoa, E., ... & Kantartzis, K. (2018). Single Nucleotide Polymorphisms in the G-Protein Coupled Receptor Kinase 5 (GRK5) Gene are associated with Plasma LDL-Cholesterol Levels in Humans. Scientific Reports, 8(1), 7745.

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For research use only. Not intended for any clinical use.

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