Mouse Anti-GTF2B Recombinant Antibody (CBLG1-2102) (CBMAB-G5673-LY)

Name: Mouse Anti-GTF2B Recombinant Antibody (CBLG1-2102)
SKU: CBMAB-G5673-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-2102

Application

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

general transcription factor IIB

Introduction

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	2959
Monkey	694770

UniProt ID

Human	Q00403
Monkey	F7HU28

Function

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).

Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).

Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).

Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).

Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).

Modulates transcription start site selection (PubMed:10318856).

Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

Biological Process

DNA-templated transcription, initiation Source: GO_Central
Positive regulation of core promoter binding Source: GO_Central
Protein acetylation Source: UniProtKB
RNA polymerase II core complex assembly Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: UniProtKB
Transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
Transcription by RNA polymerase II Source: ARUK-UCL
Transcription initiation from RNA polymerase II promoter Source: UniProtKB
Viral transcription Source: GO_Central

Cellular Location

Nucleus; Chromosome. Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171).

PTM

Acetylated (PubMed:24441171). Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).

Zhang, S., Lai, T., Su, X., Zhang, Y., Zhou, J., Zhao, C., ... & Hu, H. (2023). Linc00662 m6A promotes the progression and metastasis of pancreatic cancer by activating focal adhesion through the GTF2B-ITGA1-FAK pathway. American Journal of Cancer Research, 13(5), 1718.

Cai, F., Chen, S., Yu, X., Zhang, J., Liang, W., Zhang, Y., ... & Wu, Q. (2022). Transcription factor GTF2B regulates AIP protein expression in growth hormone-secreting pituitary adenomas and influences tumor phenotypes. Neuro-oncology, 24(6), 925-935.

Ruan, Z., Deng, H., Liang, M., Xu, Z., Lai, M., Ren, H., ... & Su, X. (2021). Overexpression of long non-coding RNA00355 enhances proliferation, chemotaxis, and metastasis in colon cancer via promoting GTF2B-mediated ITGA2. Translational Oncology, 14(1), 100947.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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