Mouse Anti-GTF2H5 Recombinant Antibody (M80517) (CBMAB-G5719-LY)

Name: Mouse Anti-GTF2H5 Recombinant Antibody (M80517)
SKU: CBMAB-G5719-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

M80517

Application

Immunogen

TFIIH antibody was raised in Rabbit using a purified recombinant human TFIIH protein fragments expressed in Ecoli as the immunogen

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

general transcription factor IIH, polypeptide 5

Entrez Gene ID

404672

UniProt ID

Q6ZYL4

Alternative Names

General Transcription Factor IIH Subunit 5; TFIIH Basal Transcription Factor Complex TTD-A Subunit; General Transcription Factor IIH; Polypeptide 5; TFB5 Ortholog; C6orf175; TTDA; TFIIH Basal Transcription Factor Complex TTDA Subunit; DNA Repair Syndrome Trichothiodystrophy Group A; General Transcription Factor IIH Polypeptide 5; Chromosome 6 Open Reading Frame 175;

Function

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell.

Biological Process

Cellular response to gamma radiation Source: Ensembl
Maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: Ensembl
Nucleotide-excision repair Source: MGI
Nucleotide-excision repair, preincision complex assembly Source: GO_Central
Phosphorylation of RNA polymerase II C-terminal domain Source: GO_Central
Transcription by RNA polymerase II Source: GO_Central
Transcription elongation from RNA polymerase I promoter Source: Ensembl
Transcription initiation from RNA polymerase II promoter Source: InterPro

Cellular Location

Nucleus; Cytoplasm

Involvement in disease

Trichothiodystrophy 3, photosensitive (TTD3):
A form of trichothiodystrophy, an autosomal recessive disease characterized by sulfur-deficient brittle hair and multisystem variable abnormalities. The spectrum of clinical features varies from mild disease with only hair involvement to severe disease with cutaneous, neurologic and profound developmental defects. Ichthyosis, intellectual and developmental disabilities, decreased fertility, abnormal characteristics at birth, ocular abnormalities, short stature, and infections are common manifestations. There are both photosensitive and non-photosensitive forms of the disorder.

Michalska, E., Koppolu, A., Dobrzańska, A., Płoski, R., & Gruszfeld, D. (2019). A case of severe trichothiodystrophy 3 in a neonate due to mutation in the GTF2H5 gene: clinical report. European Journal of Medical Genetics, 62(9), 103557.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-GTF2H5 Recombinant Antibody (CBLG1-2124) (CAT#: CBMAB-G5720-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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