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Rabbit Anti-GZMB Recombinant Antibody (D2H2F) (CBMAB-CP0868-LY)

The product is antibody recognizes GZMB. The antibody D2H2F immunoassay techniques such as: WB,IP,IF,IF (ICC),FC.
See all GZMB antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
D2H2F
Antibody Isotype
IgG
Application
WB, IP, IF, IF (ICC), FC

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Granzyme B protein.
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Granzyme B
Introduction
This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Entrez Gene ID
Human3002
Mouse14939
UniProt ID
HumanP10144
MouseP04187
Alternative Names
Granzyme B; T-Cell Serine Protease 1-3E; Cathepsin G-Like 1; Granzyme B (Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1); Cytotoxic T-Lymphocyte Proteinase 2; Cytotoxic Serine Protease B; Human Lymphocyte Protein; Fragmentin 2; EC 3.4.21.79; CTSGL1; CTLA1; CSPB; CGL1; SECT;
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927).

It cleaves after Asp (PubMed:8258716, PubMed:1985927).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257).

Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).
Biological Process
Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of translation Source: CACAO
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB
Cellular Location
Cytolytic granule; Secreted. Delivered into the target cell by perforin (PubMed:20038786).

Gleave, A., & Granville, D. J. (2023). Granzyme B in Autoimmune Skin Disease. Biomolecules, 13(2), 388.

Tibbs, E., & Cao, X. (2022). Emerging canonical and non-canonical roles of granzyme B in health and disease. Cancers, 14(6), 1436.

Wang, H., Huang, Y., He, J., Zhong, L., & Zhao, Y. (2021). Dual roles of granzyme B. Scandinavian Journal of Immunology, 94(3), e13086.

Turner, C. T., Zeglinski, M. R., Richardson, K. C., Santacruz, S., Hiroyasu, S., Wang, C., ... & Granville, D. J. (2021). Granzyme B contributes to barrier dysfunction in oxazolone-induced skin inflammation through E-cadherin and FLG cleavage. Journal of Investigative Dermatology, 141(1), 36-47.

Turner, C. T., Bolsoni, J., Zeglinski, M. R., Zhao, H., Ponomarev, T., Richardson, K., ... & Granville, D. J. (2021). Granzyme B mediates impaired healing of pressure injuries in aged skin. npj Aging and Mechanisms of Disease, 7(1), 6.

Xie, M. M., Fang, S., Chen, Q., Liu, H., Wan, J., & Dent, A. L. (2019). Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells. JCI insight, 4(16).

Turner, C. T., Lim, D., & Granville, D. J. (2019). Granzyme B in skin inflammation and disease. Matrix Biology, 75, 126-140.

Arabpour, M., Rasolmali, R., Talei, A. R., Mehdipour, F., & Ghaderi, A. (2019). Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes. Molecular immunology, 114, 172-178.

Chaves-Pozo, E., Valero, Y., Lozano, M. T., Rodríguez-Cerezo, P., Miao, L., Campo, V., ... & Cuesta, A. (2019). Fish granzyme A shows a greater role than granzyme B in fish innate cell-mediated cytotoxicity. Frontiers in Immunology, 10, 2579.

Russo, V., Klein, T., Lim, D. J., Solis, N., Machado, Y., Hiroyasu, S., ... & Granville, D. J. (2018). Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Scientific reports, 8(1), 9690.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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