Mouse Anti-GZMB Recombinant Antibody (GZB01) (CBMAB-G1005-LY)

Name: Mouse Anti-GZMB Recombinant Antibody (GZB01)
SKU: CBMAB-G1005-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

GZB01

Application

IHC-P

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Granzyme B

Introduction

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Entrez Gene ID

3002

UniProt ID

P10144

Alternative Names

Granzyme B; T-Cell Serine Protease 1-3E; Cathepsin G-Like 1; Granzyme B (Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1); Cytotoxic T-Lymphocyte Proteinase 2; Cytotoxic Serine Protease B; Human Lymphocyte Protein; Fragmentin 2; EC 3.4.21.79; CTSGL1; CTLA1; CSPB; CGL1; SECT;

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927).

It cleaves after Asp (PubMed:8258716, PubMed:1985927).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257).

Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).

Biological Process

Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of translation Source: CACAO
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB

Cellular Location

Cytolytic granule; Secreted. Delivered into the target cell by perforin (PubMed:20038786).

Gleave, A., & Granville, D. J. (2023). Granzyme B in Autoimmune Skin Disease. Biomolecules, 13(2), 388.

Tibbs, E., & Cao, X. (2022). Emerging canonical and non-canonical roles of granzyme B in health and disease. Cancers, 14(6), 1436.

Wang, H., Huang, Y., He, J., Zhong, L., & Zhao, Y. (2021). Dual roles of granzyme B. Scandinavian Journal of Immunology, 94(3), e13086.

Turner, C. T., Zeglinski, M. R., Richardson, K. C., Santacruz, S., Hiroyasu, S., Wang, C., ... & Granville, D. J. (2021). Granzyme B contributes to barrier dysfunction in oxazolone-induced skin inflammation through E-cadherin and FLG cleavage. Journal of Investigative Dermatology, 141(1), 36-47.

Turner, C. T., Bolsoni, J., Zeglinski, M. R., Zhao, H., Ponomarev, T., Richardson, K., ... & Granville, D. J. (2021). Granzyme B mediates impaired healing of pressure injuries in aged skin. npj Aging and Mechanisms of Disease, 7(1), 6.

Xie, M. M., Fang, S., Chen, Q., Liu, H., Wan, J., & Dent, A. L. (2019). Follicular regulatory T cells inhibit the development of granzyme B–expressing follicular helper T cells. JCI insight, 4(16).

Turner, C. T., Lim, D., & Granville, D. J. (2019). Granzyme B in skin inflammation and disease. Matrix Biology, 75, 126-140.

Arabpour, M., Rasolmali, R., Talei, A. R., Mehdipour, F., & Ghaderi, A. (2019). Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes. Molecular immunology, 114, 172-178.

Chaves-Pozo, E., Valero, Y., Lozano, M. T., Rodríguez-Cerezo, P., Miao, L., Campo, V., ... & Cuesta, A. (2019). Fish granzyme A shows a greater role than granzyme B in fish innate cell-mediated cytotoxicity. Frontiers in Immunology, 10, 2579.

Russo, V., Klein, T., Lim, D. J., Solis, N., Machado, Y., Hiroyasu, S., ... & Granville, D. J. (2018). Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Scientific reports, 8(1), 9690.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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