Mouse Anti-IDE Recombinant Antibody (A371) (CBMAB-AP10826LY)

Name: Mouse Anti-IDE Recombinant Antibody (A371)
SKU: CBMAB-AP10826LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A371

Application

IF, IHC, WB

Immunogen

Synthetic Peptide

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Insulin Degrading Enzyme

Introduction

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Entrez Gene ID

3416

UniProt ID

P14735

Alternative Names

Insulin Degrading Enzyme

Function

E-AB-22041

Biological Process

Amyloid-beta clearance Source: ARUK-UCL
Amyloid-beta clearance by cellular catabolic process Source: ARUK-UCL
Amyloid-beta metabolic process Source: UniProtKB
Antigen processing and presentation of endogenous peptide antigen via MHC class I Source: UniProtKB
Bradykinin catabolic process Source: UniProtKB
Cellular protein catabolic process Source: UniProtKB
Determination of adult lifespan Source: UniProtKB
Hormone catabolic process Source: UniProtKB
Insulin catabolic process Source: UniProtKB
Insulin metabolic process Source: UniProtKB
Insulin receptor signaling pathway Source: UniProtKB
Peptide catabolic process Source: UniProtKB
Positive regulation of protein binding Source: UniProtKB
Positive regulation of protein catabolic process Source: ARUK-UCL
Protein catabolic process Source: ARUK-UCL
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Regulation of aerobic respiration Source: ARUK-UCL
Ubiquitin recycling Source: UniProtKB

Cellular Location

Cytosol; Secreted; Cell membrane. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

PTM

The N-terminus is blocked.

Azam, M. S., Wahiduzzaman, M., Reyad-ul-Ferdous, M., Islam, M. N., & Roy, M. (2022). Inhibition of insulin degrading enzyme to control diabetes mellitus and its applications on some other chronic disease: A critical review. Pharmaceutical research, 39(4), 611-629.

González-Casimiro, C. M., Merino, B., Casanueva-Álvarez, E., Postigo-Casado, T., Cámara-Torres, P., Fernández-Díaz, C. M., ... & Perdomo, G. (2021). Modulation of insulin sensitivity by insulin-degrading enzyme. Biomedicines, 9(1), 86.

Leissring, M. A., González-Casimiro, C. M., Merino, B., Suire, C. N., & Perdomo, G. (2021). Targeting insulin-degrading enzyme in insulin clearance. International Journal of Molecular Sciences, 22(5), 2235.

Leissring, M. A. (2021). Insulin-degrading enzyme: Paradoxes and possibilities. Cells, 10(9), 2445.

Sousa, L., Guarda, M., Meneses, M. J., Macedo, M. P., & Vicente Miranda, H. (2021). Insulin‐degrading enzyme: an ally against metabolic and neurodegenerative diseases. The Journal of pathology, 255(4), 346-361.

Maianti, J. P., Tan, G. A., Vetere, A., Welsh, A. J., Wagner, B. K., Seeliger, M. A., & Liu, D. R. (2019). Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme. Nature chemical biology, 15(6), 565-574.

Kurochkin, I. V., Guarnera, E., & Berezovsky, I. N. (2018). Insulin-degrading enzyme in the fight against Alzheimer’s disease. Trends in pharmacological sciences, 39(1), 49-58.

Zhang, Z., Liang, W. G., Bailey, L. J., Tan, Y. Z., Wei, H., Wang, A., ... & Tang, W. J. (2018). Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme. Elife, 7, e33572.

Li, H., Wu, J., Zhu, L., Sha, L., Yang, S., Wei, J., ... & Yang, Z. (2018). Insulin degrading enzyme contributes to the pathology in a mixed model of Type 2 diabetes and Alzheimer’s disease: possible mechanisms of IDE in T2D and AD. Bioscience reports, 38(1), BSR20170862.

Villa-Pérez, P., Merino, B., Fernández-Díaz, C. M., Cidad, P., Lobatón, C. D., Moreno, A., ... & Perdomo, G. (2018). Liver-specific ablation of insulin-degrading enzyme causes hepatic insulin resistance and glucose intolerance, without affecting insulin clearance in mice. Metabolism, 88, 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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