Mouse Anti-IRS1 (Phosphorylated Y612) Recombinant Antibody (738662) (PTM-CBMAB-0089LY)

Name: Mouse Anti-IRS1 (Phosphorylated Y612) Recombinant Antibody (738662)
SKU: PTM-CBMAB-0089LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

738662

Application

Immunogen

Phosphopeptide containing the human IRS1 Y612 site, Accession # P35568

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

insulin receptor substrate 1

Introduction

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Entrez Gene ID

3667

UniProt ID

P35568

Alternative Names

Insulin Receptor Substrate 1

Function

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Biological Process

Cellular response to fatty acidISS:ARUK-UCL
Cellular response to insulin stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Glucose homeostasisManual Assertion Based On ExperimentTAS:BHF-UCL
Insulin receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Insulin-like growth factor receptor signaling pathwayManual Assertion Based On ExperimentIPI:UniProtKB
Negative regulation of insulin receptor signaling pathwayISS:BHF-UCL
Negative regulation of insulin secretionManual Assertion Based On ExperimentIDA:BHF-UCL
Phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of cell population proliferation1 PublicationNAS:BHF-UCL
Positive regulation of fatty acid beta-oxidationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of glucose importManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of glycogen biosynthetic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of insulin receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of phosphatidylinositol 3-kinase activityISS:BHF-UCL
Response to insulinManual Assertion Based On ExperimentIDA:BHF-UCL
Response to peptide hormoneISS:BHF-UCL
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Caveola; Cytoplasm; Cytosol; Insulin receptor complex; Intracellular membrane-bounded organelle; Nucleoplasm; Nucleus; Plasma membrane

Involvement in disease

Diabetes mellitus, non-insulin-dependent (NIDDM):
A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

PTM

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity).
Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).
Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (PubMed:18952604).
Phosphorylated on tyrosine residues in response to insulin (PubMed:23401856).
In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1 (PubMed:23401856).
Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).

Gao, X. K., Rao, X. S., Cong, X. X., Sheng, Z. K., Sun, Y. T., Xu, S. B., ... & Zhou, Y. T. (2022). Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discovery, 8(1), 60.

Sharma, M., Aggarwal, S., Nayar, U., Vikram, N. K., Misra, A., & Luthra, K. (2021). Differential expression of insulin receptor substrate-1 (IRS-1) in visceral and subcutaneous adipose depots of morbidly obese subjects undergoing bariatric surgery in a tertiary care center in north India; SNP analysis and correlation with metabolic profile. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 15(3), 981-986.

Dwyer, A. R., Truong, T. H., Kerkvliet, C. P., Paul, K. V., Kabos, P., Sartorius, C. A., & Lange, C. A. (2021). Insulin receptor substrate-1 (IRS-1) mediates progesterone receptor-driven stemness and endocrine resistance in oestrogen receptor+ breast cancer. British Journal of Cancer, 124(1), 217-227.

Ochiai, T., Sano, T., Nagayama, T., Kubota, N., Kadowaki, T., Wakabayashi, T., & Iwatsubo, T. (2021). Differential involvement of insulin receptor substrate (IRS)-1 and IRS-2 in brain insulin signaling is associated with the effects on amyloid pathology in a mouse model of Alzheimer's disease. Neurobiology of Disease, 159, 105510.

Albegali, A. A., Shahzad, M., Mahmood, S., & Ullah, M. I. (2019). Genetic association of insulin receptor substrate-1 (IRS-1, rs1801278) gene with insulin resistant of type 2 diabetes mellitus in a Pakistani population. Molecular biology reports, 46, 6065-6070.

Gorgisen, G., Hapil, F. Z., Yilmaz, O., Cetin, Z., Pehlivanoglu, S., Ozbudak, I. H., ... & Ozes, O. N. (2019). Identification of novel mutations of Insulin Receptor Substrate 1 (IRS1) in tumor samples of non-small cell lung cancer (NSCLC): Implications for aberrant insulin signaling in development of cancer. Genetics and molecular biology, 42, 15-25.

Piper, A. J., Clark, J. L., Mercado-Matos, J., Matthew-Onabanjo, A. N., Hsieh, C. C., Akalin, A., & Shaw, L. M. (2019). Insulin Receptor Substrate-1 (IRS-1) and IRS-2 expression levels are associated with prognosis in non-small cell lung cancer (NSCLC). PLoS One, 14(8), e0220567.

Alipourfard, I., Datukishvili, N., & Mikeladze, D. (2019). TNF-α downregulation modifies insulin receptor substrate 1 (IRS-1) in metabolic signaling of diabetic insulin-resistant hepatocytes. Mediators of Inflammation, 2019.

Zhuang, J., Song, Y., Ye, Y., He, S., Ma, X., Zhang, M., ... & Xia, W. (2019). PYCR1 interference inhibits cell growth and survival via c-Jun N-terminal kinase/insulin receptor substrate 1 (JNK/IRS1) pathway in hepatocellular cancer. Journal of translational medicine, 17, 1-10.

Rabiee, A., Krüger, M., Ardenkjær-Larsen, J., Kahn, C. R., & Emanuelli, B. (2018). Distinct signalling properties of insulin receptor substrate (IRS)-1 and IRS-2 in mediating insulin/IGF-1 action. Cellular signalling, 47, 1-15.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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