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Mouse Anti-ITK Recombinant Antibody (CBYJT-1401) (CBMAB-T0423-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to ITK (IL2 Inducible T Cell Kinase). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ITK antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1401
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Purified recombinant fragment of ITK (aa2-110) expressed in E. coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 2-110

Target

Full Name
IL2 Inducible T Cell Kinase
Introduction
ITK is an intracellular tyrosine kinase expressed in T-cells. ITK contains both SH2 and SH3 domains which are often found in intracellular kinases. ITK is thought to function in T-cell proliferation and differentiation.
Entrez Gene ID
UniProt ID
Alternative Names
IL2 Inducible T Cell Kinase
Function
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281).
Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity).
Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).
Biological Process
Activation of phospholipase C activityISS:UniProtKB
Adaptive immune responseISS:UniProtKB
B cell receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Cellular defense responseManual Assertion Based On ExperimentTAS:ProtInc
Gamma-delta T cell activationISS:UniProtKB
Intracellular signal transductionIEA:InterPro
NK T cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Peptidyl-tyrosine autophosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytokine productionISS:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
T cell activationManual Assertion Based On ExperimentTAS:UniProtKB
T cell receptor signaling pathwayISS:UniProtKB
Cellular Location
Cytoplasm; Nucleus. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
Involvement in disease
Lymphoproliferative syndrome 1 (LPFS1):
A rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
PTM
Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
Ubiquitinated.

Martinez-Planes, E., Fontela, M. G., Ramello, C., & Abate-Daga, D. (2023). CD28-Y218 phosphorylation in CAR-T cells is mediated by the IL-2-inducible T-cell kinase (ITK). Cancer Research, 83(7_Supplement), 1770-1770.

Liang, W., Li, K., Zhang, Q., Li, K., Ai, K., Zhang, J., ... & Yang, J. (2022). Interleukin-2 inducible T cell kinase (ITK) may participate in the anti-bacterial immune response of Nile tilapia via regulating T-cell activation. Fish & Shellfish Immunology, 127, 419-426.

Mammadli, M., Harris, R., Suo, L., May, A., Gentile, T., Waickman, A. T., ... & Karimi, M. (2021). Interleukin‐2‐inducible T‐cell kinase (Itk) signaling regulates potent noncanonical regulatory T cells. Clinical and Translational Medicine, 11(12), e625.

Huang, L., Ye, K., McGee, M. C., Nidetz, N. F., Elmore, J. P., Limper, C. B., ... & Huang, W. (2020). Interleukin-2-Inducible T-cell kinase deficiency impairs early pulmonary protection against Mycobacterium tuberculosis infection. Frontiers in immunology, 10, 3103.

Lechner, K. S., Neurath, M. F., & Weigmann, B. (2020). Role of the IL-2 inducible tyrosine kinase ITK and its inhibitors in disease pathogenesis. Journal of molecular medicine, 98, 1385-1395.

Mammadli, M., Huang, W., Harris, R., Sultana, A., Cheng, Y., Tong, W., ... & Karimi, M. (2020). Targeting interleukin-2-inducible T-cell kinase (ITK) differentiates GVL and GVHD in allo-HSCT. Frontiers in immunology, 11, 593863.

Elmore, J. P., McGee, M. C., Nidetz, N. F., Anannya, O., Huang, W., & August, A. (2020). Tuning T helper cell differentiation by ITK. Biochemical Society Transactions, 48(1), 179-185.

Mamontov, P., Eberwine, R. A., Perrigoue, J., Das, A., Friedman, J. R., & Mora, J. R. (2019). A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiation. PLoS One, 14(4), e0215963.

Hain, A., Krämer, M., Linka, R. M., Nakhaei-Rad, S., Ahmadian, M. R., Häussinger, D., ... & Münk, C. (2018). IL-2 inducible kinase ITK is critical for HIV-1 infection of Jurkat T-cells. Scientific Reports, 8(1), 3217.

Mamand, S., Allchin, R. L., Ahearne, M. J., & Wagner, S. D. (2018). Comparison of interleukin-2-inducible kinase (ITK) inhibitors and potential for combination therapies for T-cell lymphoma. Scientific reports, 8(1), 14216.

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For research use only. Not intended for any clinical use.

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