Mouse Anti-JMY Recombinant Antibody (A726) (CBMAB-AP4049LY)

Name: Mouse Anti-JMY Recombinant Antibody (A726)
SKU: CBMAB-AP4049LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A726

Application

IHC, WB

Immunogen

Synthetic peptide from the c terminus of the peptide sequence, identical for both isoform 1 and 2

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

junction mediating and regulatory protein, p53 cofactor

Entrez Gene ID

133746

UniProt ID

Q8N9B5

Function

35-152747

Biological Process

de novo' actin filament nucleationISS:UniProtKB
Actin polymerization-dependent cell motilityISS:UniProtKB
Arp2/3 complex-mediated actin nucleationISS:UniProtKB
Cellular response to starvationManual Assertion Based On ExperimentIMP:UniProtKB
DNA repairIEA:UniProtKB-KW
Intrinsic apoptotic signaling pathway by p53 class mediatorISS:UniProtKB
Positive regulation of apoptotic processISS:UniProtKB
Positive regulation of DNA-binding transcription factor activityISS:UniProtKB
Regulation of transcription by RNA polymerase IIISS:HGNC-UCL

Cellular Location

Nucleus; Cytoplasmic vesicle; Cytoplasm, cytoskeleton; Endomembrane system; Cytoplasmic vesicle, autophagosome membrane. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity).
In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells. Localizes to cytoplasmic vesicles which associate with actin filament and autophagosomal membranes upon starvation-induced autophagy (PubMed:30420355).

PTM

Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response (By similarity).

Rodriguez-Pastrana, I., Birli, E., & Coutts, A. S. (2023). p53-dependent DNA repair during the DNA damage response requires actin nucleation by JMY. Cell Death & Differentiation, 1-12.

Ke, X., Yang, R., Wu, F., Wang, X., Liang, J., Hu, X., & Hu, C. (2021). Exosomal miR-218-5p/miR-363-3p from endothelial progenitor cells ameliorate myocardial infarction by targeting the p53/JMY signaling pathway. Oxidative medicine and cellular longevity, 2021.

Gauthier, L. R., Saati, M., Bensalah-Pigeon, H., Ben M’Barek, K., Gitton-Quent, O., Bertrand, R., ... & Boussin, F. D. (2020). The HIF1α/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation. Scientific Reports, 10(1), 18742.

Zuo, W., Guo, W. S., Yu, H. C., Liu, P., & Zhang, Q. D. (2020). Role of Junction‐Mediating and Regulatory Protein in the Pathogenesis of Glucocorticoid‐Induced Endothelial Cell Lesions. Orthopaedic Surgery, 12(3), 964-973.

Lin, Z. L., Li, Y. H., Jin, Y. X., & Kim, N. H. (2019). A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse. Development & Reproduction, 23(3), 285.

Liu, X., & Klionsky, D. J. (2019). Regulation of JMY’s actin nucleation activity by TTC5/STRAP and LC3 during autophagy. Autophagy, 15(3), 373-374.

Purnell, M. C. (2019). Bio-Field Array: The Influence of Junction Mediating and Regulatory Protein Expression on Cytoskeletal Filament Behavior During Apoptosis in Triple-Negative Breast Cancer. Breast Cancer: Basic and Clinical Research, 13, 1178223419830981.

Adighibe, O., & Pezzella, F. (2018). The role of JMY in p53 regulation. Cancers, 10(6), 173.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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