Summary
Basic Information
Immunogen
KCNE1 (1 a.a. ~ 106 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
Formulations & Storage [For reference only, actual COA shall prevail!]
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Target
Introduction
The product of this gene belongs to the potassium channel KCNE family. Potassium ion channels are essential to many cellular functions and show a high degree of diversity, varying in their electrophysiologic and pharmacologic properties. This gene encodes a transmembrane protein known to associate with the product of the KVLQT1 gene to form the delayed rectifier potassium channel. Mutation in this gene are associated with both Jervell and Lange-Nielsen and Romano-Ward forms of long-QT syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Alternative Names
Potassium Voltage-Gated Channel Subfamily E Regulatory Subunit 1; Potassium Channel; Voltage Gated Subfamily E Regulatory Beta Subunit 1; IKs Producing Slow Voltage-Gated Potassium Channel Subunit Beta Mink; Potassium Voltage-Gated Channel; Isk-Related Family; Member 1; Delayed Rectifier Potassium Channel Subunit IsK; Minimal Potassium Channel; Potassium Voltage-Gated Channel; Isk-Related Subfamily; Member 1; Potassium Voltage-Gated Channel Subfamily E Member 1; Cardiac Delayed Rectifier Potassium Channel Protein;
Function
Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19219384).
Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).
Biological Process
Cardiac muscle cell action potential involved in contractionManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to cAMPManual Assertion Based On ExperimentIDA:BHF-UCL
Membrane repolarizationManual Assertion Based On ExperimentIDA:BHF-UCL
Membrane repolarization during action potentialManual Assertion Based On ExperimentIDA:BHF-UCL
Membrane repolarization during cardiac muscle cell action potentialManual Assertion Based On ExperimentIDA:BHF-UCL
Membrane repolarization during ventricular cardiac muscle cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of delayed rectifier potassium channel activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein targeting to membraneISS:BHF-UCL
Positive regulation of potassium ion transmembrane transportManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion export across plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion transmembrane transportManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of delayed rectifier potassium channel activityManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of heart rate by cardiac conductionManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of potassium ion transmembrane transportManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of ventricular cardiac muscle cell membrane repolarizationManual Assertion Based On ExperimentIMP:BHF-UCL
Sensory perception of soundManual Assertion Based On ExperimentTAS:ProtInc
Ventricular cardiac muscle cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular Location
Cell membrane; Apical cell membrane; Membrane raft. Colocalizes with KCNB1 at the plasma membrane (By similarity).
Targets to the membrane raft when associated with KCNQ1 (PubMed:20533308).
Involvement in disease
Jervell and Lange-Nielsen syndrome 2 (JLNS2):
An autosomal recessive disorder characterized by congenital deafness, prolongation of the QT interval, syncopal attacks due to ventricular arrhythmias, and a high risk of sudden death.
Long QT syndrome 5 (LQT5):
A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
Topology
Helical: 44-66
Cytoplasmic: 67-129
PTM
Phosphorylation inhibits the potassium current.
N-glycosylation at Asn-26 oCcurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.
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Datasheet