Summary
Basic Information
Immunogen
Purified recombinant fragment of human LEF1 (AA: 33-138) expressed in E. Coli.
Clonality
Monoclonal Antibody
Application Notes
| Application | Note |
| WB | 1:500-1:2,000 |
| IHC-P | 1:200-1:1,000 |
| ELISA | 1:10,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Target
Function
Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090).
Participates in the Wnt signaling pathway (By similarity).
Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity).
PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).
TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540).
Regulates T-cell receptor alpha enhancer function (PubMed:19653274).
Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity).
May play a role in hair cell differentiation and follicle morphogenesis (By similarity).
Isoform 1
Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Isoform 3
Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.
Isoform 5
Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
Biological Process
Anatomical structure regressionIEA:Ensembl
Apoptotic process involved in blood vessel morphogenesisIEA:Ensembl
B cell proliferationIEA:Ensembl
BMP signaling pathwayIEA:Ensembl
Branching involved in blood vessel morphogenesisIEA:Ensembl
Canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Cell chemotaxisManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to cytokine stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to interleukin-4Manual Assertion Based On ExperimentIDA:UniProtKB
Chorio-allantoic fusionIEA:Ensembl
Dentate gyrus developmentIEA:Ensembl
Embryonic limb morphogenesisIEA:Ensembl
Epithelial to mesenchymal transitionISS:BHF-UCL
Face morphogenesisIEA:Ensembl
Forebrain neuroblast divisionIEA:Ensembl
Forebrain radial glial cell differentiationIEA:Ensembl
Formation of radial glial scaffoldsIEA:Ensembl
Histone H3 acetylationManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K56 acetylationIEA:Ensembl
Histone H4 acetylationManual Assertion Based On ExperimentIMP:UniProtKB
Mammary gland developmentIEA:Ensembl
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of apoptotic process in bone marrow cellManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cell-cell adhesionIDA:UniProtKB
Negative regulation of DNA bindingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of interleukin-13 productionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of interleukin-4 productionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of interleukin-5 productionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of striated muscle tissue developmentIEA:Ensembl
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Neutrophil differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Odontogenesis of dentin-containing toothIEA:Ensembl
Osteoblast differentiationManual Assertion Based On ExperimentIEP:UniProtKB
Paraxial mesoderm formationIEA:Ensembl
Positive regulation by host of viral transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell cycle processIDA:UniProtKB
Positive regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell proliferation in bone marrowManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of cell-cell adhesionIDA:UniProtKB
Positive regulation of chondrocyte proliferationIEA:Ensembl
Positive regulation of epithelial to mesenchymal transitionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of gamma-delta T cell differentiationISS:UniProtKB
Positive regulation of gene expressionManual Assertion Based On ExperimentIDA:AgBase
Positive regulation of granulocyte differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of Wnt signaling pathwayIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Secondary palate developmentISS:BHF-UCL
Sensory perception of tasteIEA:Ensembl
SomitogenesisIEA:Ensembl
Sprouting angiogenesisIEA:Ensembl
T cell receptor V(D)J recombinationIEA:Ensembl
T-helper 1 cell differentiationISS:UniProtKB
Tongue developmentIEA:Ensembl
Trachea gland developmentIEA:Ensembl
Cellular Location
Nucleus
Found in nuclear bodies upon PIASG binding.
PTM
Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.
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Datasheet