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Mouse Anti-mCherry Antibody (3A11) (CBMAB-1421CQ)

This product is a mouse antibody that recognizes mCherry. The antibody 3A11 can be used for immunoassay techniques such as: WB.
See all mCherry antibodies

Summary

Host Animal
Mouse
Specificity
Discosoma, All species
Clone
3A11
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
Recombinant mCherry expressed in E. coli
Specificity
Discosoma, All species
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
mCherry fluorescent protein
Introduction
mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 nm and has an emission maximum at 583 nm. Recombinant mCherry is expressed and purified from transformed E. coli. The protein is a 28.8 kDa monomer of 236 amino acids with a peak fluorescent excitation and emission at 587 nm and 610 nm, respectively. It is stable and resistant to photobleaching. It matures quickly, with a t0.5 of 15 minutes, allowing it to be visualised soon after translation.
Alternative Names
mCherry; mCherry fluorescent protein
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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