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Mouse Anti-MHCA Recombinant Antibody (21-55-4) (CBMAB-0090-CN)

This product is a mouse antibody that recognizes MHCA of Dictyostelium discoideum. The antibody 21-55-4 can be used for immunoassay techniques such as: WB.
See all MHCA antibodies

Summary

Host Animal
Mouse
Specificity
D. discoideum, Dictyostelium
Clone
21-55-4
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Myosin II heavy chain
Specificity
D. discoideum, Dictyostelium
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Myosin II heavy chain
Introduction
Myosin II heavy chain, component of the actin-based molecular motor (conventional myosin); has actin-activated ATPase activity; myosin II consists of two heavy chains and one essential (mlcE) and one regulatory light chain (mlcR), respectively. Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
Entrez Gene ID
UniProt ID
Alternative Names
MHC
Function
Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
Biological Process
Actin-myosin filament sliding Source: dictyBase
Aggregation involved in sorocarp development Source: dictyBase
Bleb assembly Source: dictyBase
Cell motility Source: dictyBase
Chemotaxis Source: dictyBase
Contractile actin filament bundle assembly Source: dictyBase
Contractile vacuole organization Source: dictyBase
Cortical actin cytoskeleton organization Source: dictyBase
Culmination involved in sorocarp development Source: dictyBase
Cytoplasmic actin-based contraction involved in forward cell motility Source: dictyBase
Detection of mechanical stimulus Source: dictyBase
Filopodium assembly Source: dictyBase
Hypotonic response Source: dictyBase
Mitotic cytokinesis Source: dictyBase
Myosin filament assembly Source: dictyBase
Negative regulation of actin filament polymerization Source: dictyBase
Protein localization Source: dictyBase
Pseudopodium retraction Source: dictyBase
Regulation of cell shape Source: dictyBase
Response to cAMP Source: dictyBase
Response to differentiation-inducing factor 1 Source: dictyBase
Response to hydrogen peroxide Source: dictyBase
Response to mechanical stimulus Source: dictyBase
Uropod retraction Source: dictyBase
Cellular Location
Cell cortex
Note: Highest concentration in the posterior cell cortex.
PTM
Phosphorylation inhibits thick filament formation and reduces the actin-activated ATPase activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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