Mouse Anti-MHCA Recombinant Antibody (21-55-4) (CBMAB-0090-CN)

Name: Mouse Anti-MHCA Recombinant Antibody (21-55-4)
SKU: CBMAB-0090-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

21-55-4

Application

Immunogen

Myosin II heavy chain

Specificity

D. discoideum, Dictyostelium

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Myosin II heavy chain

Introduction

Myosin II heavy chain, component of the actin-based molecular motor (conventional myosin); has actin-activated ATPase activity; myosin II consists of two heavy chains and one essential (mlcE) and one regulatory light chain (mlcR), respectively. Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Entrez Gene ID

8625606

UniProt ID

P08799

Alternative Names

MHC

Function

Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Biological Process

Actin-myosin filament sliding Source: dictyBase
Aggregation involved in sorocarp development Source: dictyBase
Bleb assembly Source: dictyBase
Cell motility Source: dictyBase
Chemotaxis Source: dictyBase
Contractile actin filament bundle assembly Source: dictyBase
Contractile vacuole organization Source: dictyBase
Cortical actin cytoskeleton organization Source: dictyBase
Culmination involved in sorocarp development Source: dictyBase
Cytoplasmic actin-based contraction involved in forward cell motility Source: dictyBase
Detection of mechanical stimulus Source: dictyBase
Filopodium assembly Source: dictyBase
Hypotonic response Source: dictyBase
Mitotic cytokinesis Source: dictyBase
Myosin filament assembly Source: dictyBase
Negative regulation of actin filament polymerization Source: dictyBase
Protein localization Source: dictyBase
Pseudopodium retraction Source: dictyBase
Regulation of cell shape Source: dictyBase
Response to cAMP Source: dictyBase
Response to differentiation-inducing factor 1 Source: dictyBase
Response to hydrogen peroxide Source: dictyBase
Response to mechanical stimulus Source: dictyBase
Uropod retraction Source: dictyBase

Cellular Location

Cell cortex
Note: Highest concentration in the posterior cell cortex.

PTM

Phosphorylation inhibits thick filament formation and reduces the actin-activated ATPase activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-MHCA Recombinant Antibody (21-96-3) (CAT#: CBMAB-0091-CN)

Isotype control

For research use only. Not intended for any clinical use.

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