Mouse Anti-MLF1 Recombinant Antibody (CBFYM-2283) (CBMAB-M2468-FY)

Name: Mouse Anti-MLF1 Recombinant Antibody (CBFYM-2283)
SKU: CBMAB-M2468-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2283

Application

FC, IF, IHC-P, WB

Immunogen

Full length human recombinant protein of human MLF1(NP_071888) produced in E. coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.93 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MYELOID LEUKEMIA FACTOR 1

Introduction

This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4291

UniProt ID

P58340

Alternative Names

Myeloid Leukemia Factor 1; Myelodysplasia-Myeloid Leukemia Factor 1; Myeloid Leukemia Factor 1 Variant 1; Myeloid Leukemia Factor 1 Variant 2; Myeloid Leukemia Factor 1 Variant 3

Function

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

Biological Process

Cell cycle Source: UniProtKB-KW
Myeloid progenitor cell differentiation Source: UniProtKB
Regulation of cell cycle G1/S phase transition Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: UniProtKB
Regulation of transcription, DNA-templated Source: GO_Central
Transcription, DNA-templated Source: UniProtKB

Cellular Location

Nucleus
Cytoskeleton
cilium basal body
Cytoplasm
Other locations
cilium
Note: Shuttles between the cytoplasm and nucleus.

Involvement in disease

A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.

PTM

Phosphorylation is required for binding to YWHAZ.

Li, Z., Yang, Y., Wu, K., Li, Y., & Shi, M. (2023). Myeloid leukemia factor 1: A “double-edged sword” in health and disease. Frontiers in Oncology, 13, 1124978.

Yang, Z., Zhang, H., Xu, M. Y., Zhang, X. F., Wang, Y. Y., Ge, S. T., ... & Zuo, L. G. (2023). Prognostic Value of the Expression of Myeloid Leukemia Factor 1-Interacting Protein in Gastric Cancer and Its Regulatory Role in Tumor Progression. Sichuan da xue xue bao. Yi xue ban= Journal of Sichuan University. Medical science edition, 54(1), 114-121.

Tang, Z., Yang, Y., Chen, W., & Liang, T. (2023). Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signaling. Hepatology Communications, 7(8), e0204.

Wu, J. H., Tung, S. Y., Ho, C. C., Su, L. H., Gan, S. W., Liao, J. Y., ... & Sun, C. H. (2021). A myeloid leukemia factor homolog involved in encystation-induced protein metabolism in Giardia lamblia. Biochimica et Biophysica Acta (BBA)-General Subjects, 1865(6), 129859.

Ito, H., Nakamae, I., Kato, J. Y., & Yoneda-Kato, N. (2021). Stabilization of fatty acid synthesis enzyme acetyl-CoA carboxylase 1 suppresses acute myeloid leukemia development. The Journal of Clinical Investigation, 131(12).

Huang, D., Liu, C., Sun, X., Sun, X., Qu, Y., Tang, Y., ... & Tong, T. (2020). CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochemical and Biophysical Research Communications, 529(2), 127-132.

Higo, J., Kawabata, T., Kusaka, A., Kasahara, K., Kamiya, N., Fukuda, I., ... & Nakamura, H. (2020). Molecular interaction mechanism of 14-3-3 ε protein with phosphorylated Myeloid leukemia factor 1 revealed by an enhanced conformational sampling. bioRxiv, 2020-05.

Darracq, A., Pak, H., Bourgoin, V., Zmiri, F., Dellaire, G., Affar, E. B., & Milot, E. (2019). NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes. PLoS genetics, 15(11), e1008463.

Shiba, N., Yoshida, K., Hara, Y., Yamato, G., Shiraishi, Y., Matsuo, H., ... & Hayashi, Y. (2019). Transcriptome analysis offers a comprehensive illustration of the genetic background of pediatric acute myeloid leukemia. Blood advances, 3(20), 3157-3169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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