Mouse Anti-MMP2 Recombinant Antibody (A1145) (CBMAB-AP4416LY)

Mouse

Human

A1145

IgG1

ELISA, IHC, WB

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP2 protein and mouse myeloma Ag8563 cells.

Human

IgG1

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

Affinity purity

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Matrix Metallopeptidase 2; Matrix Metalloproteinase-2; EC 3.4.24.24; CLG4A; MMP-2; TBE-1; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metalloproteinase-II; 72 KDa Type IV Collagenase;

X2054M

Aging Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Blood vessel maturation Source: Ensembl
Bone trabecula formation Source: Ensembl
Cell migration Source: Ensembl
Cellular protein metabolic process Source: Reactome
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to estradiol stimulus Source: Ensembl
Cellular response to fluid shear stress Source: Ensembl
Cellular response to interleukin-1 Source: Ensembl
Cellular response to reactive oxygen species Source: Ensembl
Cellular response to UV-A Source: UniProtKB
Collagen catabolic process Source: GO_Central
Embryo implantation Source: Ensembl
Endodermal cell differentiation Source: UniProtKB
Ephrin receptor signaling pathway Source: Reactome
Extracellular matrix disassembly Source: ParkinsonsUK-UCL
Extracellular matrix organization Source: GO_Central
Face morphogenesis Source: Ensembl
Heart development Source: Ensembl
Intramembranous ossification Source: Ensembl
Luteinization Source: Ensembl
Negative regulation of cell adhesion Source: Ensembl
Negative regulation of vasoconstriction Source: Ensembl
Ovarian follicle development Source: Ensembl
Ovulation from ovarian follicle Source: Ensembl
Parturition Source: Ensembl
Peripheral nervous system axon regeneration Source: Ensembl
Positive regulation of apoptotic process Source: Ensembl
Positive regulation of cell migration Source: Ensembl
Positive regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Prostate gland epithelium morphogenesis Source: Ensembl
Proteolysis Source: ParkinsonsUK-UCL
Response to activity Source: Ensembl
Response to amyloid-beta Source: ARUK-UCL
Response to electrical stimulus Source: Ensembl
Response to estrogen Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to hyperoxia Source: Ensembl
Response to hypoxia Source: GO_Central
Response to mechanical stimulus Source: Ensembl
Response to nicotine Source: Ensembl
Response to retinoic acid Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl
Tissue remodeling Source: GO_Central

Isoform 1:
Nucleus
Extracellular region or secreted
extracellular matrix
Other locations
Membrane
Note: Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Isoform 2:
Cytoplasm
Mitochondrion

Multicentric osteolysis, nodulosis, and arthropathy (MONA):
An autosomal recessive syndrome characterized by severe multicentric osteolysis with predominant involvement of the hands and feet. Additional features include coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy.

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.