Recombinant Mouse Anti-MOAP1 Recombinant Antibody (4A12) (CBMAB-XB0831-YC)

Name: Recombinant Mouse Anti-MOAP1 Recombinant Antibody (4A12)
SKU: CBMAB-XB0831-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4A12

Application

IF, WB

Immunogen

GST-MOAP-1 (N-terminal) fusion protein

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Modulator Of Apoptosis 1

Introduction

MOAP1 was identified by its interaction with apoptosis regulator BAX protein. This protein contains a Bcl-2 homology 3 (BH3)-like motif, which is required for the association with BAX. When overexpressed, this gene has been shown to mediate caspase-dependent apoptosis.

Entrez Gene ID

64112

UniProt ID

Q96BY2

Alternative Names

Modulator Of Apoptosis 1; Paraneoplastic Ma Antigen Family Member 4; Paraneoplastic Antigen Ma4; MAP-1; PNMA4; MAP1; Paraneoplastic Antigen Like 4;

Function

Retrotransposon-derived protein that forms virion-like capsids (By similarity).

Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525).

Required for death receptor-dependent apoptosis (PubMed:11060313).

When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439).

Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314).

Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215).

Biological Process

Apoptotic signaling pathway Source: BHF-UCL
Extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
Intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of autophagosome assembly Source: UniProtKB
Positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: BHF-UCL
Regulation of apoptotic process Source: GO_Central

Cellular Location

Mitochondrion
Mitochondrion outer membrane
cytosol 1 Publication
Other locations
Extracellular vesicle membrane
Note: Forms virion-like extracellular vesicles that are released from cells.

PTM

Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39.

Koong, X., Look, K., Abdullah, A. D. I., Tan, K. O., Mani, V., Ser, H. L., ... & Ming, L. C. (2023). Therapeutic targeting of MOAP-1 in cancer: A systematic review of current approaches and future directions. Progress In Microbes & Molecular Biology, 6(1).

Zurowska, K., Alam, A., Ganser‐Pornillos, B. K., & Pornillos, O. (2022). Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins. Proteins: Structure, Function, and Bioinformatics, 90(1), 309-313.

Su, Y., Wang, W., & Meng, X. (2022). Revealing the Roles of MOAP1 in Diseases: A Review. Cells, 11(5), 889.

Liu, L., Liu, B., Zhang, B., Ye, Y., & Jiang, W. (2022). Polystyrene micro (nano) plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis. Journal of Hazardous Materials, 438, 129550.

Simon, S. E., Lim, H. S., Jayakumar, F. A., Tan, E. W., & Tan, K. O. (2022). Alpha-Mangostin activates MOAP-1 tumor suppressor and mitochondrial signaling in MCF-7 human breast cancer cells. Evidence-Based Complementary and Alternative Medicine, 2022, 1-12.

Tan, C. T., Chang, H. C., Zhou, Q., Yu, C., Fu, N. Y., Sabapathy, K., & Yu, V. C. (2021). MOAP‐1‐mediated dissociation of p62/SQSTM1 bodies releases Keap1 and suppresses Nrf2 signaling. EMBO reports, 22(1), e50854.

Chang, H. C., Tao, R. N., Tan, C. T., Wu, Y. J., Bay, B. H., & Yu, V. C. (2021). The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore. Autophagy, 17(11), 3725-3739.

Pandeya, A., Khalko, R. K., Mishra, A., Singh, N., Singh, S., Saha, S., ... & Gosipatala, S. B. (2021). Human Cytomegalovirus miR-UL70-3p Downregulates the H2O2-Induced Apoptosis by Targeting the Modulator of Apoptosis-1 (MOAP1). International Journal of Molecular Sciences, 23(1), 18.

Lee, Y. H., Pang, S. W., Revai Lechtich, E., Shah, K., Simon, S. E., Ponnusamy, S., ... & Tan, K. O. (2020). Tricistronic expression of MOAP-1, Bax and RASSF1A in cancer cells enhances chemo-sensitization that requires BH3L domain of MOAP-1. Journal of cancer research and clinical oncology, 146, 1751-1764.

Guo, X. Y., Li, Y., Fan, J., Xiong, H., Xu, F. X., Shi, J., ... & Wang, W. M. (2019). Host-induced gene silencing of MoAP1 confers broad-spectrum resistance to Magnaporthe oryzae. Frontiers in Plant Science, 10, 433.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MOAP1 Matched Antibody Pair (742) (CAT#: APMAB-742LY)

MOAP1 Matched Antibody Pair (743) (CAT#: APMAB-743LY)

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MOAP1 Matched Antibody Pair (743) (CAT#: APMAB-743LY)

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Isotype control

For research use only. Not intended for any clinical use.

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