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Recombinant Mouse Anti-MOAP1 Recombinant Antibody (4A12) (CBMAB-XB0831-YC)

Provided herein is a Mouse Recombinant Antibody against Modulator Of Apoptosis 1. The antibody can be used for immunoassay techniques, such as IF, WB.
See all MOAP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4A12
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Immunogen
GST-MOAP-1 (N-terminal) fusion protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Modulator Of Apoptosis 1
Introduction
MOAP1 was identified by its interaction with apoptosis regulator BAX protein. This protein contains a Bcl-2 homology 3 (BH3)-like motif, which is required for the association with BAX. When overexpressed, this gene has been shown to mediate caspase-dependent apoptosis.
Entrez Gene ID
64112
UniProt ID
Q96BY2
Alternative Names
Modulator Of Apoptosis 1; Paraneoplastic Ma Antigen Family Member 4; Paraneoplastic Antigen Ma4; MAP-1; PNMA4; MAP1; Paraneoplastic Antigen Like 4;
Function
Retrotransposon-derived protein that forms virion-like capsids (By similarity).

Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525).

Required for death receptor-dependent apoptosis (PubMed:11060313).

When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439).

Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314).

Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215).
Biological Process
Apoptotic signaling pathway Source: BHF-UCL
Extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
Intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of autophagosome assembly Source: UniProtKB
Positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: BHF-UCL
Regulation of apoptotic process Source: GO_Central
Cellular Location
Mitochondrion
Mitochondrion outer membrane
cytosol 1 Publication
Other locations
Extracellular vesicle membrane
Note: Forms virion-like extracellular vesicles that are released from cells.
PTM
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39.

Koong, X., Look, K., Abdullah, A. D. I., Tan, K. O., Mani, V., Ser, H. L., ... & Ming, L. C. (2023). Therapeutic targeting of MOAP-1 in cancer: A systematic review of current approaches and future directions. Progress In Microbes & Molecular Biology, 6(1).

Zurowska, K., Alam, A., Ganser‐Pornillos, B. K., & Pornillos, O. (2022). Structural evidence that MOAP1 and PEG10 are derived from retrovirus/retrotransposon Gag proteins. Proteins: Structure, Function, and Bioinformatics, 90(1), 309-313.

Su, Y., Wang, W., & Meng, X. (2022). Revealing the Roles of MOAP1 in Diseases: A Review. Cells, 11(5), 889.

Liu, L., Liu, B., Zhang, B., Ye, Y., & Jiang, W. (2022). Polystyrene micro (nano) plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis. Journal of Hazardous Materials, 438, 129550.

Simon, S. E., Lim, H. S., Jayakumar, F. A., Tan, E. W., & Tan, K. O. (2022). Alpha-Mangostin activates MOAP-1 tumor suppressor and mitochondrial signaling in MCF-7 human breast cancer cells. Evidence-Based Complementary and Alternative Medicine, 2022, 1-12.

Tan, C. T., Chang, H. C., Zhou, Q., Yu, C., Fu, N. Y., Sabapathy, K., & Yu, V. C. (2021). MOAP‐1‐mediated dissociation of p62/SQSTM1 bodies releases Keap1 and suppresses Nrf2 signaling. EMBO reports, 22(1), e50854.

Chang, H. C., Tao, R. N., Tan, C. T., Wu, Y. J., Bay, B. H., & Yu, V. C. (2021). The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore. Autophagy, 17(11), 3725-3739.

Pandeya, A., Khalko, R. K., Mishra, A., Singh, N., Singh, S., Saha, S., ... & Gosipatala, S. B. (2021). Human Cytomegalovirus miR-UL70-3p Downregulates the H2O2-Induced Apoptosis by Targeting the Modulator of Apoptosis-1 (MOAP1). International Journal of Molecular Sciences, 23(1), 18.

Lee, Y. H., Pang, S. W., Revai Lechtich, E., Shah, K., Simon, S. E., Ponnusamy, S., ... & Tan, K. O. (2020). Tricistronic expression of MOAP-1, Bax and RASSF1A in cancer cells enhances chemo-sensitization that requires BH3L domain of MOAP-1. Journal of cancer research and clinical oncology, 146, 1751-1764.

Guo, X. Y., Li, Y., Fan, J., Xiong, H., Xu, F. X., Shi, J., ... & Wang, W. M. (2019). Host-induced gene silencing of MoAP1 confers broad-spectrum resistance to Magnaporthe oryzae. Frontiers in Plant Science, 10, 433.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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