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Mouse Anti-MR1 Recombinant Antibody (5E9) (CBMAB-A5575-LY)

The product is antibody recognizes MR1. The antibody 5E9 immunoassay techniques such as: WB, ELISA.
See all MR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5E9
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MR1 (NP_001522, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
major histocompatibility complex, class I-related
Entrez Gene ID
3140
UniProt ID
Q95460
Alternative Names
HLALS
Function
Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells (PubMed:23051753, PubMed:26795251, PubMed:12794138, PubMed:19416870, PubMed:22692454, PubMed:23846752).

In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers (PubMed:26795251, PubMed:24695216, PubMed:20581831).

Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively (PubMed:24695216).

May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin (PubMed:27527800, PubMed:31113973).

Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells (PubMed:24695216, PubMed:27527800, PubMed:23846752).

During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora (By similarity).

Acts as an immune sensor of cancer cell metabolome (PubMed:31959982).

May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types (PubMed:31959982).
Biological Process
Antigen processing and presentation of exogenous antigen Source: UniProtKB
Antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB-KW
Defense response to Gram-negative bacterium Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Immune response Source: ProtInc
Innate immune response Source: UniProtKB-KW
Positive regulation of T cell mediated cytotoxicity directed against tumor cell target Source: UniProtKB
T cell differentiation in thymus Source: UniProtKB
Cellular Location
Plasma membrane
Cell membrane
Golgi apparatus
Golgi apparatus membrane
Endoplasmic reticulum
Endoplasmic reticulum membrane
Endosome
Early endosome membrane
Late endosome membrane
Note: In the absence of antigen remains within the endoplasmic reticulum where it acts as a metabolite sensor. Antigen binding triggers trafficking of the ternary complex to the plasma membrane. After presentation, most of these complexes are rapidly internalized and degraded via endocytosis. A small subset recycles via endosomes back to the plasma membrane and may thus acquire and present new antigens that do not efficiently reach the endoplasmic reticulum.
Isoform 1:
Plasma membrane
Cell membrane
Endoplasmic reticulum
Endoplasmic reticulum membrane
Isoform 3:
Plasma membrane
Cell membrane
Endoplasmic reticulum
Endoplasmic reticulum membrane
Note: The larger proportion remains in the ER in an immature state. The subset that reach cell surface does it through a B2M-independent pathway.
Isoform 4:
Secreted
Topology
Extracellular: 23-302
Helical: 303-323
Cytoplasmic: 324-341
PTM
N-glycosylated.

Purohit, S. K., Samer, C., McWilliam, H. E., Traves, R., Steain, M., McSharry, B. P., ... & Slobedman, B. (2023). Varicella Zoster Virus Impairs Expression of the Nonclassical Major Histocompatibility Complex Class I–Related Gene Protein (MR1). The Journal of Infectious Diseases, 227(3), 391-401.

McWilliam, H. E., & Villadangos, J. A. (2023). MR1 antigen presentation to MAIT cells and other MR1-restricted T cells. Nature Reviews Immunology, 1-15.

Wang, C. J., Awad, W., Liu, L., Mak, J. Y., Veerapen, N., Illing, P. T., ... & Le Nours, J. (2022). Quantitative affinity measurement of small molecule ligand binding to major histocompatibility complex class-I–related protein 1 MR1. Journal of Biological Chemistry, 298(12).

Kulicke, C. A., De Zan, E., Hein, Z., Gonzalez-Lopez, C., Ghanwat, S., Veerapen, N., ... & Salio, M. (2022). The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation. Journal of Biological Chemistry, 298(2).

Crowther, M. D., & Sewell, A. K. (2021). The burgeoning role of MR1-restricted T-cells in infection, cancer and autoimmune disease. Current Opinion in Immunology, 69, 10-17.

Van Rhijn, I., & Le Nours, J. (2021). CD1 and MR1 recognition by human γδ T cells. Molecular immunology, 133, 95-100.

Kulicke, C., Karamooz, E., Lewinsohn, D., & Harriff, M. (2020). Covering all the bases: Complementary MR1 antigen presentation pathways sample diverse antigens and intracellular compartments. Frontiers in immunology, 11, 2034.

Le Nours, J., Gherardin, N. A., Ramarathinam, S. H., Awad, W., Wiede, F., Gully, B. S., ... & Rossjohn, J. (2019). A class of γδ T cell receptors recognize the underside of the antigen-presenting molecule MR1. Science, 366(6472), 1522-1527.

Plasil, M., Wijkmark, S., Elbers, J. P., Oppelt, J., Burger, P. A., & Horin, P. (2019). The major histocompatibility complex of Old World camelids: class I and class I‐related genes. Hla, 93(4), 203-215.

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For research use only. Not intended for any clinical use.

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